Standardized bovine colostrum derivative impedes development of type 1 diabetes in rodents |
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| Institution: | 1. Department of Immunology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Serbia;2. Department of Biomedical Sciences, School of Medicine, University of Catania, Catania, Italy;1. Department of Paediatrics, Foundation IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy;2. Department of Maternal and Children’s Health, Obstetrics and Gynecology Unit, Foundation IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy;3. Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Italy;4. Department of Maternal and Children’s Health, Neonatal Intensive Care Unit, Foundation IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy;1. Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea;2. Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea;1. Department of Toxicology, School of public health, Zhejiang University School of Medicine, Hangzhou 310058, China;2. Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China;3. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;4. The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 31000, China;5. Department of Clinical Medicine, Zhejiang University City College School of Medicine, Hangzhou 310000, China;6. Blood center of Zhejiang Province, Hangzhou 310000, China;1. Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, 800 Rose Street Rm MS409, Lexington, KY 40536, USA;2. Department of Pharmacy Practice and Science, University of Kentucky College of Pharmacy, 789 S. Limestone Street Suite 292, Lexington, KY 40536, USA;3. Department of Internal Medicine, University of Kentucky College of Medicine, 900 S. Limestone Street Suite 303, Lexington, KY 40536, USA |
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| Abstract: | Bovine colostrum is a rich source of nutrients and immunologically active components that play a role in conveying passive immunity to the offspring, protection and maturation of new-born’s gastrointestinal tract. Colostrum has exerted positive effects in diseases affecting gastrointestinal tract, as well as type 2 diabetes (T2D). However, health-promoting effects in type 1 diabetes have not been reported. The aim of this study was to investigate therapeutic value of oral administration of standardized bovine colostrum derivative (SBCD) in three models of type 1 diabetes (T1D): spontaneously developed T1D in NOD mice and BB-DP rats, and in chemically induced T1D in C57BL/6 mice with multiple low doses of streptozotocin (MLDS). SBCD was administered per os and the disease development was evaluated by weekly measurement of blood glucose and by histological analyses of the pancreas. SBCD administration prevented diabetes development in all three models, as indicated by euglicaemia. Ex vivo analysis of cytokine expression and production in the spleen and mesenteric lymph nodes (MLN) in MLDS challenged mice revealed a strong modulation of the immune response. In the MLN cells SBCD disrupted harmful Th17 response induced by MLDS. Expression of Th1 signature cytokine IFN-γ was down-regulated in MLN cells of SBCD-treated mice, while IL-4 secretion (Th2 cytokine) was up-regulated in comparison to diabetic group. Modulation of the immune response seen in the MLN protruded to the spleen, giving overall less infiltration of immune cells to the pancreas. SBCD acted on immune cells and halted (auto) aggression towards pancreatic beta cells. Moreover, SBCD induced beta cell proliferation. Hence, this derivative could be tested in diabetes and other similar diseases with aberrant immune response. |
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| Keywords: | Beta cell Bovine colostrum Inflammation T lymphocytes Type 1 diabetes |
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