| 反相高效液相色谱法检测齐多夫啶的血药浓度 |
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| 引用本文： | 高署,李俊,戎隆富,金涌,姚宏伟,过林,徐叔云. 反相高效液相色谱法检测齐多夫啶的血药浓度[J]. 中国药理学通报, 2002, 18(6): 707-709 |
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| 作者姓名： | 高署 李俊 戎隆富 金涌 姚宏伟 过林 徐叔云 |
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| 作者单位： | 1. 安徽医科大学临床药理研究所、药学系,合肥,230032
2. 安徽省卫生防疫站,合肥,230001 |
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| 基金项目： | 卫生部科学研究基金资助课题 No 98 1 336 |
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| 摘 要： | 目的 建立齐多夫啶血药浓度的反相高效液相色谱检测方法。方法 以shim pack ,VP ODS 5 μm 4 6× 15 0mm为分离柱 ,流动相∶四氢呋喃∶水∶三氟酸 (10∶89 8∶0 2 ) ,流速 1 0ml·min-1;检测波长 2 67nm。结果 齐多夫啶浓度在 0 0 2～ 2 0mg·L-1范围内与峰面积呈良好的线性关系 ,日内及日间差均小于 5 % ,平均回收率为 96 4% ,血浆中最低检测极限为 10 μg·L-1。结论 本方法具有快速、灵敏、稳定的特点 ,能满足血药浓度检测的需要
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| 关 键 词： | HPLC 齐多夫啶 血药浓度 |
| 文章编号： | 1001-1978(2002)06-0707-03 |
R-HPLC determination of plasma AZT concentration |
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| GAO Shu,LI Jun,RONG Long Fu,JIN Yong,YAO HongWei,GUO Lin,XU ShuYun. R-HPLC determination of plasma AZT concentration[J]. Chinese Pharmacological Bulletin, 2002, 18(6): 707-709 |
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| Authors: | GAO Shu LI Jun RONG Long Fu JIN Yong YAO HongWei GUO Lin XU ShuYun |
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| Abstract: | AIM To establish a RP HPLC method for the determination of zidovudine(AZT) plasma concentration. METHODS A shim pack,VP ODS. 5 μm 4 6×150 mm radial compression column was used with a mobile phase of tetrahydrofuran water trifluoroacetic acid(10∶89 8∶0 2). The flow rate was 1 0 ml·min -1 .The detector was operated at 267 nm. RESULTS The calibration curves of AZT were linear separately within the concentration range of 0 02～20 mg·L -1 . The relative standard deviations for within day and between days assays were all less than 5%. The relative recovery of the method were 96 4%. The limit of quantitation of the assay is 10 μg·L -1 of plasma. CONCLUSIONS The methed is rapid,accurate and stable. The determination of AZT plasma concendration could be used. |
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| Keywords: | HPLC zidovudine plasma concentration |
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