益妇宁软胶囊对去势大鼠骨质疏松症的影响

目的:以去卵巢大鼠为实验对象,观察益妇宁软胶囊(YFN)对绝经后骨质疏松症的影响。方法:采用3月龄SD雌性大鼠,行双侧卵巢切除术,随机分为YFN大、中、小剂量组和阳性药对照组、模型对照组及假手术组。术后4周开始给药,给药12周后收集标本。采用放免法测定血清雌二醇水平,血清钙、磷、碱性磷酸酶含量;称量子宫重量;测量大鼠肱骨长度、横径及肱骨干重、湿重;采用原子吸收光谱法测定骨矿物质钙、镁、锌、锰含量;使用双能x线骨密度仪测定离体股骨密度。结果:VFN能明显提高去势大鼠体内雌激素水平,增加性器官重量;使去势大鼠骨长、宽、干重、湿重增加;增加骨钙、镁、锌、锰等矿物质含量;增强绝经后疏松骨质的密度。结论:YFN能起到治疗绝经后骨质疏松症的作用。

Laboratory study of effects of Yifuning soft capsules on postmenopausal osteoporosis

Objective: To study the effects of Yifuning soft capsules on postmenopausal osteoporosis in ovariectomized female rats. Method: 60 female sprague-dawley rats in 3-month were used,50 of them were ovariectomized and randomly divided into 5 groups: ovariecto-my(OVX), OVX with diethylstilbestrol tables(DT), OVX with YFN(high dose, middle dose and low dose), the others were sham-operated group. Began to give the rats drugs in the fifth week after the operation. After 12 weeks killed the rats. The blood, the uterus and bones were collected to inspect. The content of estrogen(E₂) in serum was detected by radioimmunoassay method. The contents of Ca,P, ALP in serum were detected using the automic biochemistry-analyse device. The uterus were weight and the length, width, dry weight and net weight of the shoulder bone were measured. The contents of Ca, Mg, Zn, Mn in the bone were measured using the automic absorption spectrophotometer. The right femer and the third lumbar vertebra were detected for BMD using the dual-energy X-ray absorptiometry scanner and compression test using electronic testing device. Result: After using the drugs, the content of E2, Ca in serum, the weight of uterus, the contents of Ca, Mg, Zn, Mn in the bone and the BMD of right femer increased significantly,the content of P, ALP in serum decreased significantly. Conclusion: Yifuning soft capsules has a good effect on postmenopausal osteoporosis, which provides evidence for clinical use.