枸杞多糖对小鼠睾丸细胞DNA损伤的保护作用

目的 :研究枸杞多糖 (LBP)对过氧化氢 (H2 O2 )引起的睾丸细胞DNA氧化损伤的保护作用。方法 :睾丸细胞先用不同浓度LBP作用 1h ,再加入 10 0 μmol LH2 O2 共同培养 2 5min ,然后用单细胞凝胶电泳技术(SCGE)测定DNA链断裂情况 ,分析LBP的抗氧化损伤作用。结果 :H2 O2 作用一定时间后 ,可引起DNA链断裂。 50、10 0、2 0 0、40 0 μg mL的LBP预处理可使H2 O2 染毒细胞的拖尾百分率和尾长显著降低。结论 :LBP本身没有致氧化损伤作用 ,它能够清除自由基 ,对氧化应激所致的睾丸细胞DNA损伤具有抑制作用

Study on protective action of lycium barbarum polysaccharides on DNA imparments of testicle cells in mice

To investigate the protective effect of lycium barbarum polysaccharides (LBP) on DNA oxidative damage of testicle cells induced by hydrogen peroxide (H2O2). The single cell gel electrophoresis(SCGE) was used to detect the breakage of DNA strand and analyze LBP protection against oxidation damage in testicle cells treated by different concentrations of LBP for 1 hour firstly, and then cultured with 100 mumol/L H2O2 for 25 min. The results showed that a certain moment action by H2O2 could induce the breakage of DNA strand. The pretreatment of LBP (50, 100, 200, 400 micrograms/ml) significantly decreased the frequencies of cells with tail moment and the tail length of testicle cells treated by H2O2. It is suggested that LBP itself could not cause the oxidation damage, but it could clean out the free-radical and restrain the DNA damage of testicle cells caused by the oxidative stress.