2型糖尿病患者血清叶黄素与体内氧化应激指标的关系

 目的 研究2型糖尿病（diabetes mellitus，DM）患者血清叶黄素含量与氧化应激反应的关系。方法 检测99例2型DM患者和83例正常对照者的空腹血糖、血清总胆固醇、血清三酰甘油；血清叶黄素、β-胡萝卜素、番茄红素和维生素E含量及血清SOD、GSH-Px、MDA、IL-6含量，采用t检验、单因素相关分析、逐步多元线性回归等方法进行统计分析。结果 2型DM患者组血清叶黄素、番茄红素、β-胡萝卜素、SOD、GSH-Px水平明显低于正常对照组（P<0.05），血清MDA、IL-6水平高于正常对照组（P<0.01）。血清叶黄素、番茄红素和β-胡萝卜素与血清SOD和GSH-Px的相关系数为正值（P<0.05），与MDA相关系数为负值（P<0.05），叶黄素、β-胡萝卜素与IL-6的相关系数为负值（P<0.05）。多因素分析表明，控制BMI和年龄因素后，血清叶黄素与MDA、IL-6含量呈负相关，与血清SOD、GSH-Px呈正相关（P<0.05）。结论 随着2型DM患者血清叶黄素水平的增加，血清MDA、IL-6含量降低，血清SOD与GSH-Px的活性增高。提示叶黄素可能通过抑制机体的氧化应激反应，降低IL-6的表达，从而减轻IL-6水平过高所导致的负面效应。

Relationship between serum lutein and oxidative stress in patients with type 2 diabetes mellitus

Objective To investigate the serum lutein level and oxidative stress in patients with type 2 diabetes mellitus (DM). Methods The concentration of fasting serum glucose, triglyceride and total cholesterol, serum lutein, lycopene, β-carotene, vitamin E, and the activity of serum superoxide dismutase (SOD), serum glutathione peroxidase (GSH-Px), and serum malondialdehyde (MDA) level in 99 patients with type 2 diabetes and 83 healthy subjects were measured. Results Compared with the healthy subjects, the average level of lutein, lycopene, β-carotene, SOD, GSH-Px in DM group decreased significantly (P<0.05), the MDA, IL-6 level increased significantly (P<0.01). R values of Pearson correlation coefficients between serum lutein, lycopene, β-carotene and SOD and GSH-Px were positive, R values of Pearson correlation coefficients between lutein, lycopene, β-carotene and MDA were negative (P<0.05), R values of Pearson correlation coefficients between serum lutein, β-carotene and IL-6 were negative (P<0.05). The results of multiple linear regression analysis showed that after adjusted by BMI and age, lutein was negatively correlated with serum MDA and IL-6, and positively correlated with SOD and GSH-Px (P<0.05). Conclusions There were close relationship between the serum biomarkers indicative of oxidative stress and serum carotenoids in patients with type 2 diabetes mellitus. As serum lutein level increased,the serum MDA and IL-6 level decreased, while SOD and GSH-Px increased, which demonstrated that lutein can decrease oxidative stress, potentially decrease IL-6 expression, and alleviate the side effects of excessive IL-6 expression.