经皮穿刺激光心肌血管建术后细胞外信号调节激酶的动脉变化

目的：探讨经皮穿刺激光心肌血管重建术（ＰＴＭＲ）后细胞外信号调节激酶的动脉变化。方法：实验组和对照组分别在猪心脏上进行ＰＴＭＲ和假手术，于ＰＴＭＲ和假手术后２、２４ｈ，３、７、１４ｄ取左室前壁心肌组织，分别以放射免疫法和Ｗｅｓｔｅｒｎ ｂｌｏｔ方法测定其细胞外信号调节激酶（ＥＲＫｓ）活性和相对蛋白含量。结果：与假手术对照组比较，ＰＴＭＲ实验组心组织内ＥＲＫｓ于２ｈ开始激活，持续至２４ｈ；其蛋白含量于术后２４ｈ开始升高并持续至术后７ｄ。结论：ＰＴＭＲ激活ＥＲＫｓ并使其蛋白合成增加。

Dynamic alternation of extracellular signal-regulated kinases induced by percutaneous transluminal myocardial laser revascularization

Objective To investigate dynamic alternation of extracellular signal regulated kinases induced by percutaneous transluminal myocardial laser revascularization (PTMR). Methods PTMR was performed on in vivo porcine hearts. Biopsies were taken from the anterior wall of the hearts treated with PTMR (or sham operation) at 2, 24 hours and 3, 7, 14 days after operation. The activity and relative protein abunance of extracellular signal regulated kinases (ERKs) were measured by radioimmunological and Western blotting methods, respectively. Results The ERKs in myocardium treated with PTMR were activated at 2 hours and lasted to 24 hours, and the relative protein abundance of ERKs increased to a peak level from 24 hours to 7 days after operation, compared with sham operated controls. Conclusion PTMR activated ERKs and upregulates the ERKs protein synthesis.