川芎提取物质量标准的研究

目的:建立川芎提取物中阿魏酸的含量测定方法,完善川芎提取物的质量标准。方法:应用反相高效液相色谱法(HPLC),色谱柱Inertsil ODS-SP;流动相为乙腈:水:冰醋酸(19∶81∶1.6);流速:1mL/min;检测波长:321nm。结果:反相高效液相色谱法方法学考察良好,阿魏酸在19.960~39.920μg/mL(r=0.9992)范围有良好的线性关系,平均回收率为102.69%(n=6,RSD=1.67%)。结论:以阿魏酸作为指标含量对川芎提取物进行质量标准控制的方法,适用于川芎提取物的质量标准控制。

Study on Quality Standard for Ligusticum chuanxiong Hort Extract

Objective： To establish a high performance liquid chromatography （HPLC）method for assaying ferulic acid in ligusticum chuangxiong Hort extract and to improve the quality standard for chuanxiong extract. Methods： The fcrulie acid was separated on a reversed-phase Cts（Inertsil ODS-SP）column using acetonitrile ：water：acetic acid （19：81：1.6 v/v/v）as the mobile phase with a flow rate of 1mL/min.The external standard method was used for quantification of ferulic acid and the detective wavelength was 321 nm. Results： The HPLC method was accurate and repro- ducible, showing good linearity over a range of 19.960μg/mL-39.920μg/mL （r=0.9992）.The average recovery was 102.69%（n=6,RSD=l.67%）. Conclusion： The method of assaying fernlic acid in ligusticum chuanxiong Hort extract can be applied for qualitative control of ligusticum chuanxiong Hort extract.