伤痛宁片对大鼠急性软组织损伤的治疗作用及其机制研究

目的研究伤痛宁片对大鼠急性软组织损伤的治疗效果及其可能机制。方法建立大鼠急性软组织损伤模型,随机分为6组,分别为对照组,模型组,云南白药组（0.25 g/kg）,伤痛宁片低、中、高剂量（0.3、0.6、1.2 g/kg）组。治疗7 d后,评估大鼠的损伤康复情况,进行病理组织学检查,分别检测超氧化物歧化酶（SOD）的活性,以及丙二醛（MDA）、一氧化氮（NO）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的含量。结果伤痛宁片中、高剂量组对大鼠急性软组织损伤程度均有显著的修复作用（P〈0.05）。伤痛宁片中、高剂量组大鼠血清SOD活性均有明显的提高,MDA含量显著降低（P〈0.05）。大鼠肌肉组织中IL-1β、NO和TNF-α均表现出不同程度的降低。结论伤痛宁片对大鼠急性软组织损伤具有治疗作用,其机制可能与伤痛宁片的抗炎和抗氧化作用有关。

Therapeutical effects and its mechanism of Shangtongning Tablets on acute soft tissue injury in rats

Objective To investigate the therapeutical effects and mechanism of Shangtongning Tablets on the acute soft tissue injury in rat models. Methods Rat models with acute soft tissue injury were established and randomly divided into normal, model, Yunnan Baiyao（0.25 g/kg）, low-, mid-, and high-dose Shangtongning Tablets（0.3, 0.6, and 1.2 g/kg） groups. After 7 d, the influence of Shangtongning Tablets on injury degree of rats and histopathology were observed. In addition, the activity of superoxide dismutase（SOD） and the levels of malonaldehyde（MDA）, nitric oxide（NO）, interleukin-1β（IL-1β）, and tumor necrosis factor α（TNF-α） were detected. Results The mid- and high-dose Shangtongning Tablets had favorable improvement of therapeutical effects on the acute soft tissue injury in rat models（P〈0.05）. The SOD activity of rats in the mid- and high-dose Shangtongning Tablets groups were increased significantly, but MDA levels were decreased significantly. Shangtongning Tablets showed inhibitory function on the levels of IL-1β, NO, and TNF-α in muscles. Conclusion Shangtongning Tablets possess therapeutical effects on acute soft tissue injury in rats, and this may be one of the mechanisms in regard to the anti-inflammatory properties and anti-oxidant activity.