Restriction endonuclease analysis ofStaphylococcus aureus plasmid DNA from three continents |
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| Authors: | B N Doebbeling M A Pfaller R J Hollis L D Boyken A C Pignatari L A Herwaldt R P Wenzel |
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| Institution: | (1) Department of Internal Medicine, The University of Iowa College of Medicine, 52242 Iowa City, Iowa, USA;(2) Department of Pathology, The University of Iowa College of Medicine, 52242 Iowa City, Iowa, USA;(3) Veterans Affairs Medical Center, 52240 Iowa City, Iowa, USA;(4) Escola Paulista de Medicina, Sao Paulo, Brazil;(5) Programs of Hospital Epidemiology and Quality Assurance, The University of Iowa Hospitals and Clinics, 52242 Iowa City, Iowa, USA |
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| Abstract: | Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains ofStaphylococcus aureus from the local region as controls when molecular typing methods are performed. |
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