| 血管生成抑素的原核表达及纯化 |
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| 引用本文： | 傅赞,吴月,李艳丽,朱自路,赵翰林,陈丙莺. 血管生成抑素的原核表达及纯化[J]. 南京医科大学学报(自然科学版), 2001, 9(5): 403-405 |
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| 作者姓名： | 傅赞 吴月 李艳丽 朱自路 赵翰林 陈丙莺 |
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| 作者单位： | [1]南京医科大学第一附属医院外科，江苏南京210029 [2]南京医科大学生物与分子生物学系，江苏南京210029 |
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| 摘 要： | 目的：构建血管生成抑素（angiostatin)的原核表达载体。并转入大肠杆菌BL21中诱导表达并纯化。方法：设计引物扩增angiostatin的cDNA，然后亚克隆入原核表达载体pET32a，并转化入大肠杆菌BL21中诱导表达。结果：通过重组质粒酶切和测序分析等方法，筛选出重组阳性克隆，转化入大肠杆菌BL21中诱导表达，并纯化成功。结论：通过构建的原核表达。获得了纯化的angiostatin蛋白。
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| 关 键 词： | 生长因子 血管生成抑素 原核表达 纯化 肿瘤 |
| 文章编号： | 1007-4368(2001)05-0403-03 |
| 修稿时间： | 2001-03-01 |
Prokaryotic Expression of Angiostatin |
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| FU Zan,WU Yue ,LI Yan li ,ZHU Zi lu ,ZHAO Han lin,CHEN B in yin. Prokaryotic Expression of Angiostatin[J]. Acta Universitatis Medicinalis Nanjing, 2001, 9(5): 403-405 |
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| Authors: | FU Zan WU Yue LI Yan li ZHU Zi lu ZHAO Han lin CHEN B in yin |
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| Affiliation: | FU Zan,WU Yue 1,LI Yan li 1,ZHU Zi lu 1,ZHAO Han lin,CHEN B in yin 1 |
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| Abstract: | Objective:To construct the expressive vecto r of angio statin and transform into E. coli BL21(DE3), to induce the expression of recombi nant angiostatin in prokaryotic system. Methods:The cDNA of Angiosta ti n was amplified by PCR, they were subcloned into vector, and transformed into th e E. coli BL21(DE3), and induced expression of recombinant angiostatin. Re sults:The recombinant clones were picked out by the restriction endonuclea se and sequence analysis. Recombinant angiostatin was highly expressed when the st rain was induced with IPTG(1 mmol/L), and angiostatin was successfully purified. Conclusion:The recombinant angiostatin can be used in further studi es. |
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| Keywords: | growth factors angiostatin prokaryotic expressi on |
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