| 激活粒细胞对大鼠离体心脏再灌注损伤的实验研究 |
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| 引用本文： | 包伟琦 戴宁. 激活粒细胞对大鼠离体心脏再灌注损伤的实验研究[J]. 中国医科大学学报, 1995, 24(1): 21-24 |
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| 作者姓名： | 包伟琦 戴宁 |
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| 作者单位： | 中国医科大学第一临床学院心脏外科，沈阳市第四医院循环科 |
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| 摘 要： | 本实验采用大鼠离体心脏模型，探讨激活粒细胞对大鼠离体心脏再灌注损伤的作用机制。实验过程观察冠状动脉流量，冠状动脉阻力，冠状动脉流出液中乳酸脱氢酶的活性及再二醛的含量。实验完毕后对心肌进行组织学检查及超微结构观察。
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| 关 键 词： | 粒细胞 离体心脏 再灌注损伤 心肌缺血 |
Reperfused Isolated Rat Heart Injured by Activated Polymorphonuclear Leucocytes |
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| Bao Weike,Dai Ning,Zhang Xianqing,et al. Reperfused Isolated Rat Heart Injured by Activated Polymorphonuclear Leucocytes[J]. Journal of China Medical University, 1995, 24(1): 21-24 |
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| Authors: | Bao Weike Dai Ning Zhang Xianqing et al |
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| Abstract: | solated rat hearts were reperfused with phorbol myristate acetate (PMA)activated polymorphonu-clear leucocytes(PMNs)(n =10),polymorphonuclear leucocytes(PMNs)(n=10)and Krebs-Hensleit(K-H)(n=10)to observe coronary flow,coronary resistence lactate dehydrogenase(LDH),malondi-adehyde(MDA) of coronary fluid and morphologic changes by light and electronic microscope. The re-sults showed PMA-activated PMNs decreased coronary flow,increased coronary resistence,LDH andMDA. Morphologic changes also showed that PMA-activated PMNs caused severe injury. It is conclud-ed that activated PMNs cause reperfusion injury mediated by oxygen free radicals released from activat-ed-PMNs. The obstruction of microcirculation by activated PMNs increases reperfusion injury. |
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| Keywords: | polymorphonuclear leucocytes isolated rat heart reperfusion injury |
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