| 蛋白激酶C及其抑制剂在输精管平滑肌舒缩中的作用 |
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| 引用本文： | 薛海晖 赵冬梅. 蛋白激酶C及其抑制剂在输精管平滑肌舒缩中的作用[J]. 中国医科大学学报, 1995, 24(6): 557-559,563 |
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| 作者姓名： | 薛海晖 赵冬梅 |
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| 作者单位： | 基础医学院生物化学教研室 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 对输精管平滑肌中分离的蛋白激酶Ｃ（ＰＫＣ）抑制剂的生理作用进行了研究。应用放射自显影方法证明，此抑制剂对输精管平滑肌中ＰＫＣ底物－４０ＫＤ蛋白的磷酸化有明显抑制作用；并用免疫印迹电泳法确定了输精管中ＰＫＣ亚型为ε亚型。以上结果表明，输精管平滑肌中ＰＫＣ抑制剂通过与ＰＫＣ－ε的相互作用，参与对平滑肌舒张与收缩的调控，还可能参与细胞分泌等细胞运动功能。
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| 关 键 词： | 蛋白激酶C 抑制剂 亚型 磷酸化 输精管 平滑肌 |
The Action of Protein Kinase C(PKC)and Its Inhibitor in Relaxation and Contraction of Smooth Muscle in Vas Deferens |
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| Xue Haihui,Yang Yu,Yu Bingzhi,et al. The Action of Protein Kinase C(PKC)and Its Inhibitor in Relaxation and Contraction of Smooth Muscle in Vas Deferens[J]. Journal of China Medical University, 1995, 24(6): 557-559,563 |
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| Authors: | Xue Haihui Yang Yu Yu Bingzhi et al |
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| Affiliation: | Dept.of Biochemistry |
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| Abstract: | e studied the physiological significance of the inhibitor of PKC purified from smooth muscle in vasdeferens.Autoradiography showed that the inhibitor could suppress the phosphorylation of 40KD pro-tein,the substrate of PKC in smooth muscle of vas deferens.The PKC subspecies in smooth muscle ofvas deferens was determined to be by Westem blot.These results indicated that through crosstalk withPKC-,the inhibitor might take part in the regulation of contraction and relaxation of smooth muscleand might be involved in cellular functions such as secretion and protoplasmic streaming,etc. |
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| Keywords: | protein kinase C inhibitor phosphorylation |
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