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| Ghrelin对棕榈酸诱导的大鼠主动脉内皮细胞凋亡的影响 | |
| 引用本文： | 张丹,王玉霞,王威,韩玲玲,刘国良. Ghrelin对棕榈酸诱导的大鼠主动脉内皮细胞凋亡的影响[J]. 中国动脉硬化杂志, 2011, 19(9): 747-750 |
| 作者姓名： | 张丹 王玉霞 王威 韩玲玲 刘国良 |
| 作者单位： | 1. 中国医科大学一院内分泌科,辽宁省沈阳市110001;中国医科大学四院内分泌科,辽宁省沈阳市110032 2. 中国医科大学四院内分泌科,辽宁省沈阳市,110032 3. 哈尔滨医科大学附属二院内分泌科,黑龙江省哈尔滨市,150086 4. 中国医科大学一院内分泌科,辽宁省沈阳市,110001 |
| 摘 要： | 目的探讨Ghrelin能否抑制棕榈酸诱导的大鼠主动脉内皮细胞凋亡。方法大鼠主动脉内皮细胞分别在含0.3 mmol/L棕榈酸的脂性培养基中孵育24 h,培养基中加或不加Ghrelin,应用MTT法检测细胞活性,Ho-echst 33258及流式细胞仪AnnexinⅤ-FITC/PI双染法检测细胞凋亡,分光光度计检测Caspase-3活性,Western Blot检测Bcl-2和Bax表达。结果棕榈酸降低细胞活性,使细胞凋亡增加至30.03%,与对照组(5.01%)相比差异有统计学意义(P<0.01),棕榈酸增加Caspase-3活性,使Bcl-2/Bax比率下降。Ghrelin能够浓度依赖性增加细胞活性,10 nmol/L是最低有效浓度,100 nmol/L时作用最显著。Ghrelin能抑制棕榈酸诱导的内皮细胞凋亡,使凋亡率降至10.03%(P<0.05)。同时Ghrelin降低Caspase-3活性并增加Bcl-2/Bax比率。结论 Ghrelin可以抑制棕榈酸诱导的血管内皮细胞凋亡,可能在棕榈酸诱导的内皮细胞损伤中发挥保护作用。 |
| 关 键 词： | Ghrelin 棕榈酸 细胞凋亡 内皮细胞 |
| 收稿时间： | 2011-01-12 |
Effect of Ghrelin on Rat Aortic Endothelial Cells Apoptosis Induced by Palmitate |
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| ZHANG Dan,WANG Yu-Xi,WANG Wei,HAN Ling-Ling,and LIU Guo-Liang. Effect of Ghrelin on Rat Aortic Endothelial Cells Apoptosis Induced by Palmitate[J]. Chinese Journal of Arteriosclerosis, 2011, 19(9): 747-750 | |
| Authors: | ZHANG Dan WANG Yu-Xi WANG Wei HAN Ling-Ling and LIU Guo-Liang |
| Affiliation: | ZHANG Dan 1,2,WANG Yu-Xia 2,WANG Wei 3,HAN Ling-Ling 2,and LIU Guo-Liang 1(1.Department of Endocrinology,First Hospital of China Medical University,Shenyang,Liaoning 110001,2.Department of Endocrinology,Fourth Hospital of China Medical University,Liaoning 110032,3.Department of Endocrinology,Second Hospital of Haerbin Medical University,Haerbin,Heilongjiang 150086,China) |
| Abstract: | Aim To investigate whether ghrelin could inhibit apoptosis induced by palmiate in rat aortic endothelial cells.Methods Rat aortic endothelial cells were cultured in 0.3 mmol/L palmitate for 24 h with or without ghrelin.Cell viability was assessed by MTT assay.Apoptosis was detected using hoechst 33258 and annexin V-FITC/PI double staining assay.Spectrofluorometer assay was used to detect caspase-3 activity.Western Blot analysis was used to examine the expression of Bcl-2 and Bax.Results Exposure of cells to... |
| Keywords: | Ghrelin Palmitate Apoptosis Endothelial Cells |
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