血凝素样氧化型低密度脂蛋白受体1基因沉默抑制人脐静脉内皮细胞表达分形趋化因子和单核细胞趋化蛋白1

目的探讨血凝素样氧化型低密度脂蛋白受体1基因沉默后能否抑制氧化型低密度脂蛋白诱导的分形趋化因子和单核细胞趋化蛋白1的表达。方法分别用不同浓度的氧化型低密度脂蛋白与人脐静脉内皮细胞共孵育,及预先对人脐静脉内皮细胞转染pGenesil-1 LOX-1 shRNA后再用氧化型低密度脂蛋白刺激,半定量RT-PCR、Western blot及酶联免疫吸附法检测血凝素样氧化型低密度脂蛋白受体1、分形趋化因子和单核细胞趋化蛋白1的mRNA和蛋白表达。结果氧化型低密度脂蛋白能呈浓度依赖性诱导血凝素样氧化型低密度脂蛋白受体1、分形趋化因子和单核细胞趋化蛋白1的mRNA和蛋白表达(P<0.01)。用RNA干扰抑制血凝素样氧化型低密度脂蛋白受体1的表达后,显著抑制了氧化型低密度脂蛋白诱导的分形趋化因子和单核细胞趋化蛋白1的mRNA和蛋白表达(P<0.01)。结论氧化型低密度脂蛋白能呈浓度依赖性诱导人脐静脉内皮细胞中分形趋化因子和单核细胞趋化蛋白1表达;这种诱导作用可以被血凝素样氧化型低密度脂蛋白受体1基因沉默抑制。

Lectin-Like Oxidized-LDL Receptor-1 Gene Silence Suppressed Expression of Fractalkine and Monocyte Chemoattractant Protein-1 Induced By Oxidized Low-Density Lipoprotein in Human Umbilical Vein Endothelial Cells

Aim To observe effect of lectin-like oxidized low density lipoprotein(ox-LDL) receptor-1(LOX-1) gene silence on the expressions of fractalkine and monocyte chemoattractant protein-1(MCP-1) induced by ox-LDL in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were cultured and treated with ox-LDL of three different concentrations(25,50,100 mg/L respectively) for 24 hours;pGenesil-1 LOX-1 shRNA were transfected into HUVEC and then cells were treated with ox-LDL(50 mg/L).mRNA and protein expressions...