透骨消痛颗粒含药血清对兔软骨细胞增殖影响的研究

目的：探讨透骨消痛颗粒含药血清对兔软骨细胞增殖的影响。方法：取4周龄新西兰兔膝关节关节软骨Ⅱ型胶原酶消化,建立软骨细胞体外培养体系,体外培养第3代软骨细胞随机分为5组,分别加入培养液、空白血清和中药血清低、中、高剂量继续培养24、48、72 h后,采用流式细胞仪检测软骨细胞周期的变化。结果：流式细胞仪细胞周期检测显示中药血清中、高剂量能够刺激软骨细胞由G0/G1期进入S期和G2/M期,并呈现时间的依赖性,干预48 h后G0/G1期细胞比例降低,S期与G2/M期细胞之和增加,与低量组、空白组相比差异有统计学意义（P〈0.05）。结论：透骨消痛颗粒含药血清能有效促进软骨细胞的增殖。

Effects of Tougu Xiaotong Granula(TXG)-containing serum on proliferation of rabbit chondrocytes in vitro

AIM: To investigate the effects of Tou-gu Xiaotong Granula(TXG)-containing serum on proliferation of rabbit chondrocytes in vitro. METHODS: The chondrocyte was obtained from the arthrodial cartilage of 4-week-old New Zealand rabbit by collagenase Ⅱ digestion, so as to establish the system of chondrocyte culture in vitro. The 3rd passage of chondrocyte in vitro was randomly divided into five groups which were respectively cultured with medium, medium with serum and the high, medium, low doses of TXG-con-taining serum. After cultured for 24, 48 and 72 hours, the cell cycle changes were detected by the flow cytom-etry. RESULTS: The high, medium doses of TXG-containing serum could stimulate the chondrocyte through G_0/G_1 phases into S and G_2/M phases in a time-dependent manner by flow cytometry. Compared with the control and low-dose groups, the proportions of cells in G_0/G_1 phase in the high- and medium-dose groups were significantly lower, meanwhile those in S and G_2/M phases were significantly higher after 48 hours(P < 0.05). CONCLUSION: TXG- containing serum could effectively promote the cell proliferation of chondrocytes.