P120catenin在胰腺癌中的表达及其基因多态性的研究

目的 探讨P120catenin在胰腺癌中的表达及其基因T755G位点多态性与胰腺癌发病因素的关系.方法 分别应用RT-PCR及Western Blot方法检测52例胰腺癌及癌旁正常胰腺组织中P120catenin的表达水平;应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对52例胰腺癌患者及60名正常对照的P120catenin基因T755G位点基因型分析.结果 胰腺癌组P120catenin mRNA及蛋白表达与癌旁正常胰腺组相比均减少,差异有统计学意义(P=0.000,P=0.002);P120catenin mRNA异常表达更多见于低分化者(P=0.033)、淋巴转移者(P=0.004)、血管侵犯者(P=0.022)和高PTNM分期者(P=0.003).胰腺癌患者P120catenin基因T755G位点基因型频率及等位基因频率与正常组相比,差异有统计学意义(P=0.008,P=0.016).与TT基因型相比,GG基因型可增加胰腺癌发病风险(OR=2.765,95%CI为1.312～3.958).结论 P120catenin低表达与胰腺癌淋巴结转移、血管侵犯、pTNM分期密切相关,提示其可能在胰腺癌侵袭、转移过程中起重要作用.P120catenin基因T755G多态性与胰腺癌的发生风险相关,有利于胰腺癌的早期诊断与预防.

The expression of P120catenin in pancreatic carcinoma and the relationship between the T755G polymorphism of P120catenin gene and pancreatic carcinoma

Objectives To investigate the expression of P120catenin in pancreatic carcinoma and to explore the association between P120catenin gene polymorphism at T755G position and pancreatic carcinoma.Methods The expression of P120catenin in 52 cases of pancreatic carcinoma and normal pancreatic tissues on the mRNA and protein levels were evaluated by RT-PCR and Western Blot methods respectively.P120catenin gene polymorphism at 1755G position of in 52 patients and 60 healthy controls were examined by PCR-restriction fragment length polymorphism (PCR-RFLP) technique.Results The mRNA and protein expressions of P120catenin in pancreatic carcinoma tissues were significantly lower than normal pancreatic tissues (P=0.000,P=0.002).Reduced expression of P120catenin mRNA was significantly correlated with differentiated (P=0.033),lymph node metastasis (P=0.004),vascular invasion (P=0.022),and pTNM stage (P=0.003).Additionally,there were significant difference of P120catenin gene polymorphism genotypes and alleles at T755G position between patients and healthy controls(P=0.008,P=0.016).The GG genotype of P120catenin gene was associated with higher risk of incidence for pancreatic carcinoma compared with the TT genotype (OR=2.765,95% CI=1.312-3.958).Conclusions The reduced expressions of both P120 catenin mRNA and protein in pancreatic carcinoma suggest its association with pancreatic carcinoma development.Polymorphism of P120catenin gene at T755G situation might be a risk factor for pancreatic carcinoma,and it may be used to diagnosis and prevent pancreatic carcinoma early.