一种新的循环肿瘤细胞检测平台在膀胱癌诊疗中的初步应用

目的 初步探索一种新的循环肿瘤细胞(CTC)检测平台在膀胱癌诊疗中的应用.方法 选取膀胱癌患者10例为试验组,健康志愿者5例为对照组.10例患者均接受经尿道膀胱肿瘤电切术.术前分别取试验组和对照组的外周全血样本10 ml、尿液样本10 ml,术后再次取试验组的外周全血、尿液样本各10 ml.使用Celsee PREP 400TM循环肿瘤细胞检测平台分别检测所有样本中所含CTC,对比术前试验组与对照组血、尿样本中的CTC,对比试验组术前、术后的CTC.随访12个月,监测患者是否出现复发或进展.结果 试验组术前血样本中均发现CTC,中位数为4个(1~9个);对照组均未发现CTC.试验组术后血样本中,8例(80%)发现CTC,中位数为1.5个(1~4个),2例(20%)未发现CTC.膀胱癌患者术前与术后血样本中的CTC比较,差异有统计学意义(P=0.014).尿液样本因杂质堵塞微通道而无法检测样本中的CTC.随访1年,患者无疾病进展/复发生存率为80%.结论 Celsee PREP 400TM循环肿瘤细胞检测平台可有效检测血样本中的CTC,值得进一步探索其在膀胱癌诊疗中的应用.

Primary application of a novel detection platform for testing circulating tumor cell(CTC)in the diagnosis and treatment of bladder cancer

Objective To investigate the primary application of a novel detection platform for testing circulating tu-mor cell(CTC)in the diagnosis and treatment of bladder cancer.Method 10 cases with bladder cancer were included in this study as study group,another 5 healthy adults were enrolled as control group.All patients in study group underwent transurethral resection of bladder tumor. The peripheral venous blood samples and urine samples were collected before surgery for 10 ml of each,and another series of sampling were performed after surgery.Celsee PREP 400TMsystem was used to characterize the CTC in all samples,which were compared between study group and control group,as well as be-fore and after surgery.The average follow-up was 12 months,and patients were monitored for the occurrence of relapse or progression.Result CTC were observed in each of the cancer patient blood samples,with a median number of 4(1-9),while no CTC was found in any of the healthy donors.CTC were observed in 8 blood samples(80%)after surgery, with a median number of 1.5(1-4),and were not found in 2 patients(20%)after surgery.The CTC was of statistically sig-nificant difference in blood samples collected before and after surgery (P=0.014). Because the microchannels were blocked by impurities in urine samples,we failed to capture CTC from these samples.After 1-year follow-up,the progres-sion-free survival rate was 80% in this cohort of patients.Conclusion Our data suggest that CTC can be detected effi-ciently with the Celsee PREP 400TMsystem,highlighting the potential clinical value of the technology.