1型和2型糖尿病大鼠模型血清氨基酸含量比较

目的:探讨1型和2型糖尿病大鼠模型血清中氨基酸的变化,了解氨基酸代谢与糖尿病的相关性.方法:分别选取链脲佐菌素诱导的1型糖尿病大鼠模型和雄性自发性Goto-kakizaki 2型糖尿病大鼠模型作为实验组;注射同体积的枸橼酸钠混悬液的雄性Wistar大鼠分别作为2个实验组的对照组.利用液相色谱质谱联用技术测定血清中14种氨基酸水平.结果:与各自的对照组比较,2个实验组糖尿病组大鼠缬氨酸、异亮氨酸水平共同升高(P<0.05~P<0.01),谷氨酰胺、赖氨酸、精氨酸和酪氨酸的水平共同降低(P<0.05~P<0.01).结论:2组糖尿病模型动物体内支链氨基酸水平呈升高的趋势,而碱性氨基酸水平下降.缬氨酸、异亮氨酸、谷氨酰胺、赖氨酸、精氨酸等的变化为糖尿病并发症的发生提供了线索和帮助.

Comparison of serum amino acid level between type 1 and type 2 diabetic rats

Objective:To investigate the changes of serum amino acid levels in type 1 and type 2 diabetic rats,and its correlation between the amino acid metabolism and diabetes. Methods:The type 1 diabetes mellitus model induced by streptozotocin and type 2 spontaneous goto-kakizaki diabetic male rats were divided into the experiment group,and male Wistar rats injected by the same volume of sodium citrate suspension were set as the control group. The serum levels of 14 kinds of amino acid level were determined by liquid chromatography-mass spectrometry. Results:Compared with the control group,the levels of valine and isoleucine in experiment group increased(P<0. 05 to P<0. 01),and the levels of glutamine,lysine,arginine and tyrosine in experiment group decreased(P<0. 05 to P<0. 01). Conclusions:The levels of branched-chain amino acid and basic amino acid in type 2 diabetic mode increase and decrease, respectively. The change of amino acid level can provide some clues and help in the occurrence of complications.