| 基于CLP败血症小鼠中补体C5a与白细胞介素-6相关性研究 |
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| 引用本文： | 周亚妮,李可,刘丹. 基于CLP败血症小鼠中补体C5a与白细胞介素-6相关性研究[J]. 蚌埠医学院学报, 2017, 42(2). DOI: 10.13898/j.cnki.issn.1000-2200.2017.02.003 |
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| 作者姓名： | 周亚妮 李可 刘丹 |
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| 作者单位： | 西安交通大学 医学部,陕西 西安710061;商洛职业技术学院 公共基础部,陕西 商洛726000;西安交通大学 医学部,陕西 西安,710061 |
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| 基金项目： | 陕西省教育厅科研计划项目，商洛职业技术学院教科研课题 |
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| 摘 要： | 目的:探讨败血症发病机制中炎症介质C5a与细胞因子白细胞介素-6(IL-6)的关系.方法:利用经典盲肠结扎穿孔术(CLP)建立败血症动物模型,在败血症活动期,取不同时间段建模组和假手术组小鼠的腹腔灌洗液,检测补体C5a和IL-6的表达.结果:建模组小鼠的腹腔灌洗液中补体C5a和IL-6水平在建模后第4、6、12、24 h均明显高于假手术组(P<0.01);伴随病变恢复,2组实验动物体内补体C5a和IL-6的表达水平逐渐趋于正常.结论:CLP败血症小鼠发病机制中,炎症介质C5a与细胞因子IL-6的调节有一定的线性相关趋势.
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| 关 键 词： | 败血症 盲肠结肠穿孔术 C5a 白细胞介素-6 小鼠 |
Study on the correlation of complement C5 a with IL-6 in CLP septicemic mice |
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| ZHOU Ya-ni,LI Ke,LIU Dan. Study on the correlation of complement C5 a with IL-6 in CLP septicemic mice[J]. Journal of Bengbu Medical College, 2017, 42(2). DOI: 10.13898/j.cnki.issn.1000-2200.2017.02.003 |
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| Authors: | ZHOU Ya-ni LI Ke LIU Dan |
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| Abstract: | Objective:To explore the relationship between inflammatory mediator C5a and interleukin-6(IL-6) in the pathogenesis of sepsis. Methods:The animal model of sepsis was established using the cecal ligation and puncture(CLP). During the active stage of sepsis,the expressions of completement C5a and IL-6 in peritoneal lavage fluid of the different time-points model group and sham operation group were detected. Results:The levels of the complement C5a and IL-6 in peritoneal lavage fluid in model group after 4,6, 12 and 24 h of establishing model were significantly higher than those in sham group(P<0. 01). With the recovery of disease,the levels of complement C5a and IL-6 in two groups gradually returned to the normal level. Conclusions:During the pathogenesis of CLP sepsis in mice,the inflammatory mediators C5a and IL-6 have a linear correlation trend. |
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| Keywords: | sepsis cecal perforation of the colon surgery C5a interleukin-6 mice |
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