腺苷在继发性脊髓损伤中的作用及其机制

目的 通过观察大鼠脊髓损伤后内源性腺苷含量的变化，以及外源性腺苷地脊髓损伤后神经功能的影响，探讨腺苷在髓继发性损伤中的作用。方法 采用大鼠Ｔ１３脊髓腹侧压迫模型，用微透析技术每２０分钟连续收集脊髓损伤后脊髓组织细胞上液，用高效液相色谱仪紫外检测法检测细胞外液腺式的含量；伤前１４分钟蛛网膜下腔给予非特异性腺苷受体激动剂２－氯腺苷，观察伤后神经功能评分、倾斜平面临界角和组织学变化。结果 脊髓损伤后腺苷

The Effect and Mechanism of Adenosine on Secondary Spinal Cord Injury

Objective To explore the role of adenosine in secondary injury mechanism after spinal cord injury (SCI) in rats. Methods A ventral compression injury model of T 13 spinal cord was produced, and the extracellular fluids were collected consecutively every 20 min after injury by using microdialysis. The adenosine in the samples was analyzed by using a high pressure liquid chromatography (HPLC) system with ultraviolet detection. The rats received different doses of 2-Chloroadenosine(2-CADO), a nonspecific agonist of adenosine receptors, by intrathecal injection 15 min before injury. The neurological function score, inclined plane angle and histology were observed after injury. Results A significant increasing of adenosine was found immediately after spinal cord injury. The concentration of adenosine reached the peak at one hour after injury and dropped down to the basal level at two hours. There was positive correlation between the increasing of adenosine and the severity of SCI. High dose of 2-CADO can improve the neurological function significantly. Conclusion The present finding suggests that adenosine could be involved in the pathological progress of secondary spinal cord injury and might play a protective role in SCI.