碱性成纤维细胞生长因子在骨愈合过程中的表达与合成

目的 通过对骨愈合过程中内源性碱性成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ，ｂＦＧＦ）合成变化的观察，探讨其成此过程的作用，以此加深对骨生理学的认识，同时为干预骨愈合提供理论依据。方法 选用ＳＤ大鼠并将其胫骨造成骨缺损，用免疫组织化学及原位杂交技术检测ｂＦＧＦ基因和蛋白质在骨闸组织成熟过程不同时期的变化情况。结果 免疫组织化学染色显示，从骨折后４天～３人，ｂ

The Gene Expression and Protein Synthesis of Basic Fibroblast Growth Factor During Bone Healing Process: An Experimental Study

Objective To observe the changes of synthesization of basic fibroblast growth factor(bFGF) and discuss its role during bone healing process in order to improve the knowledge of bone physiology and explore the theory for intervening bone healing Methods The SD rats were chosen and their tibia were cut into incompletely bone defects as the experimented models The changes of gene and protein of bFGF within the bone callus were tested by immunohistochemistry and in situ hybridization techniques Results The immunohistochemistry results showed that the positive signals appeared in the cytoplasm of ostoblasts from 4 days to 3 weeks after bone injury, and the mesenchymal stem cells were also positively stained In situ hybridization study showed that the bFGF were intensively expressed in the osteoblasts within bone defects As the time passing by, the bone callus increased and the woven bone was replaced by lamellar bone, while the osteoblasts had no or weak expression of bFGF Conclusion Osteoblasts had the potential of synthesization of bFGF, especially in initial period of bone formation and might play an important role during the process of periosteum mesenchymal stem cells proliferation and differentiation