树舌多糖GF对siRNA干扰沉默CDK2基因表达的辅助作用 |
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引用本文: | 于水澜,于英君,张淑香,宋高臣. 树舌多糖GF对siRNA干扰沉默CDK2基因表达的辅助作用[J]. 牡丹江医学院学报, 2014, 0(1): 1-3 |
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作者姓名: | 于水澜 于英君 张淑香 宋高臣 |
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作者单位: | [1]黑龙江中医药大学黑龙江哈尔滨150040 [2]牡丹江医学院黑龙江牡丹江157011 |
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基金项目: | 黑龙江省青年科技专项(QC06C090);黑龙江省中医管理局项目(zHY06-z02);黑龙江省青年科学基金(2010001) |
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摘 要: | 目的:探讨树舌多糖GF辅助siRNA干扰沉默CDK2基因表达的作用。方法:本实验采用人肝癌细胞SMMC7721为研究对象,将中药树舌多糖GF2.5μg·mL-1与siRNA干扰序列191联合应用,采用Real-timePCR方法检测CDK2基因mRNA水平表达情况。结果:树舌多糖GF2.5μg·mL-1与191序列合用比单纯应用191序列抑制率提高3%。结论:树舌多糖GF2.5μg·mL-1与191序列合用比单纯应用191序列抑制率提高3%,表明树舌多糖GF对RNAi技术沉寂肝癌CDK2基因有一定的辅助作用。
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关 键 词: | 树舌多糖GF 肝癌细胞SMMC7721 siRNA CDK2 辅助作用 |
THE STUDY OF RNA INTERFERENCE ON CDK2 GENE EXPRESSION HEPATOCELLULAR CARCINOMA CELLS AND SAAISTANT ACTION OF GAPSGF |
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Affiliation: | Yu Shui- lan etal (Heilongjiang University of Traditional Chinese Medicine Heilongjiang Harbin 150040 ,china) |
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Abstract: | Objective:To study the effect of[Polysaceharides component GF extracted by Ganoderma applanatum (Per. ex Gray) ] assisted siRNA interference silence CDK2 gene expression. Methods: In this experiment, human hepatocellular carcinoma cells line ( SMMC7721 ) used as the research object, the traditional Chinese medicine GAPSGF 2.5μgomL-1 and siRNA interference sequence 191 was applied and was detect the level of CDK2 gene expression of mRNA. with Real- time PCR method Results: Comparing with application of siRNA segment 191 alone,the inhibition ratio can? increase 3% by use GAPSGF and siRNA segment 191 combined ap- plication, Conclusions: Comparing with application of siRNA segment 191 alone, the inhibition ratio can increase 3 % by GAPSGF and siRNA segment 190 combined application,whick demonstrates that GAPSGF has certain assistant action for the RNA interfere expres- sion of CDK2. |
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Keywords: | GAPSGF SMMC7721 siRNA CDK2 assistant action |
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