凋亡抑制蛋白Survivin在胃癌中的表达及其与bcl-2、p53蛋白相关性研究

目的：研究凋亡抑制蛋白Survivin、bcl-2、p53在胃癌组织中的表达及其与临床病理特征之间的关系，并探讨Survivin与bcl-2、p53蛋白表达的相关性。方法：采用链霉菌抗生物素蛋白-过氧化酶连接（S-P）免疫组化方法，检测Survivin、bcl-2、p53蛋白在正常胃粘膜组织及胃癌组织中的表达。结果：Survivin蛋白在正常胃粘膜组织中无表达，而在58例胃癌中41例（70．7％）表达阳性，差异有统计学意义（P〈0．01）；Survivin蛋白表达与胃癌组织病理分化程度、淋巴结转移、TNM分期相关，而与浸润程度不相关；胃癌bcl-2蛋白表达的阳性与阴性中，Survivin蛋白表达阳性率分别为81．0％（34／42）和43．8％（7／16），两者比较差异显著（P〈0．01）；p53蛋白表达的阳性和阴性中，Survivin蛋白表达阳性率分别为58．1％（18／31）和22．2％（6／27），两者比较亦有显著性差异（P〈0．05），Survivin蛋白的表达与胃癌组织中的bcl-2蛋白及p53蛋白表达密切相关。结论：Survivin蛋白异常表达可引起细胞凋亡抑制，在胃癌的发生中起一定作用，其过度表达提示胃粘膜细胞增生极度活跃；Sur-vivin蛋白表达与胃癌中bcl-2蛋白及p53蛋白的异常表达密切相关。

Expression of anti-apoptosis protein survivin, and its relationship with bcl-2,p53 in gastric cancer

Objective：To study the expression of anti-apoptosis protein survivin, in gastric cancer, and to detect its clinicopathologic character and relationship with the expression of bcl-2 and p53. Methods： Immunohistochemical SP （ streptavidin-binotin peroxidase） method was adopted to detect the survivin protein expression in normal gastric tissues （ 10 patients） and survivin, bcl-2, p53 proteins expressions in gastric cancerous tissues （58 patients）. Results：No expression of survivin protein was detected in normal gastric tissues, 41 of 58 （70. 7% ） was positive in gastric cancer （ P 〈 0. 01 ）. The positive rate of expression of survivin was related to the differentiation, lymph node metastasis, and TNM stage, but not to the invasive depth. Survivin positive cases were associated with bcl-2 expression （81.0% vs. 43.8% , P 〈0. 05） and p53 expression （58. 1% vs. 22. 2% , P 〈0. 05）. The positiverate of expression of survivin protein had relationship with the expression of bcl-2, p53 proteins in gastric cancerous tissues. Conclusion：Apoptosis inhibition by the abnormal expression of survivin protein may participate in the pathogenesis of gastric cancer. The expression of survivin protein was strongly associated with the abnormal expression of bcl-2, p53 proteins in gastric cancer.