| 染色体3p区抑癌基因在非小细胞肺癌中的甲基化状况与临床意义 |
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| 引用本文： | 宋海珠,易俊,张有为,王锐,陈龙邦.染色体3p区抑癌基因在非小细胞肺癌中的甲基化状况与临床意义[J].中国肺癌杂志,2011,14(3):233-238. |
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| 作者姓名： | 宋海珠 易俊 张有为 王锐 陈龙邦 |
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| 作者单位： | 1. 第二军医大学南京临床学院(南京军区南京总医院)肿瘤内科,南京,210002
2. 第二军医大学南京临床学院(南京军区南京总医院)心胸外科,南京,210002 |
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| 摘 要： | 背景与目的 DNA甲基化是表观遗传学的一种调控机制,染色体3p区等位基因缺失是肺癌发生中较频繁和早期的事件之一。检测染色体3p区5个典型抑癌基因DLEC1、RASSF1A、hMLH1、RARβ和FHIT在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的甲基化状况,分析其临床意义。方法取78例NSCLC患者术中癌组织及相应正常肺组织标本,采用甲基化特异性聚合酶链反应(methylation specific PCR,MSP)检测基因启动子区甲基化状况,RT-PCR和免疫组化检测DLEC1基因表达。结果 78例NSCLC组织中,DLEC1、RASSF1A、RARβ和hMLH1甲基化频率分别为41.03%、39.74%、30.77%和16.67%,与正常组织相比差异均具有统计学意义。FHIT基因在癌组织和正常组织均无甲基化。DLEC1甲基化与患者临床分期(P=0.011)和淋巴结转移相关(P=0.019),而RASSF1A、RARβ、hMLH1基因甲基化以及平均甲基化指数与临床病理特征无关联。56.41%(44/78)的NSCLC组织中发现DLEC1基因表达下调或缺失,且与启动子甲基化有关。结论 3p区抑癌基因甲基化是NSCLC发生中的重要分子事件,可能作为NSCLC早期诊断的潜在生物标记,新型抑癌基因DLEC1失活与启动子高甲基化有关。
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| 关 键 词： | 肺肿瘤 染色体 甲基化 生物标记 |
DNA Methylation of Tumor Suppressor Genes Located on Chromosome 3p in Non-small Cell Lung Cancer |
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| Haizhu SONG,Jun YI,Youwei ZHANG,Rui WANG,Longbang CHEN.DNA Methylation of Tumor Suppressor Genes Located on Chromosome 3p in Non-small Cell Lung Cancer[J].Chinese Journal of Lung Cancer,2011,14(3):233-238. |
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| Authors: | Haizhu SONG Jun YI Youwei ZHANG Rui WANG Longbang CHEN |
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| Institution: | Haizhu SONG 1,Jun YI 2,Youwei ZHANG 1,Rui WANG 1,Longbang CHEN 1 1 Department of Medical Oncology,Jinling Hospital,Nangjing Clinical School of the Second Military Medical University,PLA,Nanjing 210002,China,2 Department of Cardiothoracic Surgery |
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| Abstract: | Background and objective DNA methylation is one of the mechanisms of epigenetics.Allelic loss located on chromosome 3p happen frequently and early in non-small cell lung cancer(NSCLC) .e aim of this study is to detect the promoter methylation status of tumor suppressor genes(TSGs) located on chromosome 3p in NSCLC and to evaluate its correlation with clinicopathological features.Methods A total of 78 paired NSCLC specimens and their adjacent normal tissues were collected in the study.Promoter methylation st... |
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| Keywords: | Lung neoplasms Chromosome Methylation Biomarker |
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