| 靶向人Trb3基因shRNA真核表达载体的构建及鉴定 |
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| 引用本文： | 文浩杰,隋军,孙传政,蒋玉娥,曾木圣,李满枝,张晶,李科,李晓江,王虎. 靶向人Trb3基因shRNA真核表达载体的构建及鉴定[J]. 中国耳鼻咽喉头颈外科, 2011, 0(2): 60-62 |
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| 作者姓名： | 文浩杰 隋军 孙传政 蒋玉娥 曾木圣 李满枝 张晶 李科 李晓江 王虎 |
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| 作者单位： | 昆明医学院第三附属医院头颈外科云南省头颈肿瘤研究中心;华南肿瘤学国家重点实验室中山大学肿瘤防治中心实验研究部; |
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| 基金项目： | 国家自然科学基金(30960444); 云南省科技计划项目联合专项(2009CD186); 云南省教育厅科研项目(08C0095); 华南肿瘤学国家重点实验室开放课题(303040966014)联合资助 |
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| 摘 要： | 目的 构建并鉴定靶向果蝇同源蛋白3(tribbles-related protein 3,Trb3)基因的短发夹状RNA(short hairpin RNA,shRNA)真核表达载体,并检测其对靶基因的沉默效应.方法 根据Genbank数据库中Trb3序列,设计、合成3对互补并特异性编码其shRNA序列的寡核苷酸,克隆...
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| 关 键 词： | 头颈部肿瘤 果蝇蛋白质类 RNA干扰 脂质体 真核表达载体 |
Construction and identification of the eukaryotic expression vector expressing short hairpin RNA targeting Human Trb3 Gene |
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| WEN Haojie,SUI Jun,SUN Chuanzheng,JIANG Yu'e,ZENG Musheng,LI Manzhi,ZHANG Jing,LI Ke,LI Xiaojiang,WANG Hu. Construction and identification of the eukaryotic expression vector expressing short hairpin RNA targeting Human Trb3 Gene[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2011, 0(2): 60-62 |
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| Authors: | WEN Haojie SUI Jun SUN Chuanzheng JIANG Yu'e ZENG Musheng LI Manzhi ZHANG Jing LI Ke LI Xiaojiang WANG Hu |
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| Affiliation: | WEN Haojie1,SUI Jun1,SUN Chuanzheng1,JIANG Yu'e1,ZENG Musheng2,LI Manzhi2,ZHANG Jing1,LI Ke1,LI Xiaojiang1,WANG Hu1(1 Department of Head and Heck Surgery,the Third Affiliated Hospital of Kunming Medical University,Head and Neck Cancer Research Center of Yunnan Province,Kunming,Yunnan,650118,China,2 State Key Laboratory of Oncology in South China,Department of Experimental Research,Sun Yat-Sen University Cancer Center,Guangzhou,Guangdong,510060,China) |
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| Abstract: | OBJECTIVE To construct and identify the short hairpin RNA(shRNA)eukaryotic expression vector for targeting human Trb3 gene and observe its effect on inhibiting expression of target gene.METHODSAccording to the GenBank database,three pairs of shRNA sequences targeting human Trb3 gene were designed and synthesized.The selected nucleotides were cloned in the linearized plasmid pSUPER.retro.vector respectively,and the shRNA recombinant plasmids were identified by DNA sequencing and restriction enzyme digestion.... |
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| Keywords: | Head and Neck Neoplasms Drosophila Proteins RNA Interference Liposomes eukaryotic expression vector |
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