| 非小细胞肺癌患者hOGG1基因启动子区域突变的研究 |
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| 引用本文： | 刘霞,赵军,刘仍允,雷哲,尤嘉琮,周清华,张洪涛. 非小细胞肺癌患者hOGG1基因启动子区域突变的研究[J]. 中国肺癌杂志, 2011, 14(3): 199-204. DOI: 10.3779/j.issn.1009-3419.2011.03.04 |
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| 作者姓名： | 刘霞 赵军 刘仍允 雷哲 尤嘉琮 周清华 张洪涛 |
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| 作者单位： | 1. 苏州大学癌症分子遗传学实验室,苏州,215123
2. 天津医科大学总医院,天津市肺庙研究所,天津市肺癌转移与肿瘤微环境重点实验室,天津,300052 |
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| 基金项目： | 国家自然基金(No.30973425); 教育部“新世纪优秀人才支持计划”(No.NCET-09-0165); 江苏省自然科学基金(No.BK2008162); 江苏省教育厅“青蓝工程”; 江苏省人事厅“333工程”和天津市科技支撑计划中瑞合作重大项目(No.09ZCZDSF04100)资助~~ |
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| 摘 要： | 背景与目的 8-羟基鸟嘌呤DNA糖苷酶(8-hydroxygumine DNA glycosylase1,OGG1)是一种DNA修复酶,可以从DNA切除修复8-羟基鸟嘌呤(8-dihydro-8-oxoguanine,8-OH-G)。人类OGG1基因(hOGG1)的多态性可能会改变酶的活性而影响个体修复损伤DNA的能力,促进癌变。然而,hOGG1基因启动子区域的突变与非小细胞肺癌(non-small cell lung cancer,NSCLC)的关系尚不明晰。我们拟探讨hOGG1基因启动子区域的突变与NSCLC发生发展的潜在关系。方法选取苏州大学附属第一医院2003年1月-2005年12月新鲜手术切除的40例NSCLC组织标本,采用PCR-SSCP和直接测序的方法检测NSCLC及其对应的癌旁组织中hOGG1基因启动子区域的突变。结果在40例NSCLC患者中未发现hOGG1基因启动子区域的异常突变,但发现单核苷酸多态位点rs159153与TNM分期明显相关(P=0.008);同时发现吸烟者中淋巴结转移率明显较低(P=0.034)。结论单核苷酸多态位点rs159153和吸烟史可能对NSCLC的侵袭和转移潜在性提供预测。
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| 关 键 词： | 肺肿瘤 PCR-SSCP hOGG1基因 突变 |
Mutational Analysis of hOGG1 Gene Promoter in Patients with Non-small Cell Lung Cancer |
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| Xia LIU,Jun ZHAO,Rengyun LIU,Zhe LEI,Jiacong YOU,Qinghua ZHOU,Hong-Tao ZHANG. Mutational Analysis of hOGG1 Gene Promoter in Patients with Non-small Cell Lung Cancer[J]. Chinese journal of lung cancer, 2011, 14(3): 199-204. DOI: 10.3779/j.issn.1009-3419.2011.03.04 |
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| Authors: | Xia LIU Jun ZHAO Rengyun LIU Zhe LEI Jiacong YOU Qinghua ZHOU Hong-Tao ZHANG |
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| Affiliation: | XiaLIU,Jun ZHAO,Rengyun LIU,Zhe LEI,Jiacong YOU,Qinghua ZHOU,Hong-Tao ZHANG 1 Soochow University Laboratory of Cancer Molecular Genetics,Medical College of Soochow University,Suzhou 215123,China,2 Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment,Tianjin Lung Cancer Institute,Tianjin Medical University General Hospital,Tianjin 300052 |
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| Abstract: | Background and objective 8-hydroxygumine DNA glycosylase 1(OGG1) isa DNA repair enzyme,which can repair damaged DNA by excising 8-dihydro-8-oxoguanine(8-OH-G) .Polymorphisms in human OGG1 gene(hOGG1) may alter glycosylase activity,thereby a ects its repair to the damaged DNA,resulting in contribution to carcinogenesis.However,an association of genetic variants of hOGG1 promoter with non-small cell lung cancer(NSCLC) remains unclear.e present study aims to explore whether there are mutations in the promoter ... |
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| Keywords: | Lung neoplasms PCR-SSCP hOGG1 gene Mutation |
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