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表皮干细胞的分离及鉴定
引用本文:牛云飞,路卫,夏照帆. 表皮干细胞的分离及鉴定[J]. 第二军医大学学报, 2004, 25(8): 0890-0891
作者姓名:牛云飞  路卫  夏照帆
作者单位:第二军医大学长海医院烧伤科,上海,200433;第二军医大学长海医院烧伤科,上海,200433;第二军医大学长海医院烧伤科,上海,200433
摘    要:表皮干细胞(epiderimal stem cells,ESCs)在体内数量很少,且缺乏特异性标志物,分离鉴定存在一定困难.目前,分离ESCs主要根据细胞动力学特点,以及特异性标志物标记后经流式细胞术分选来完成,已发现多种ESCs特异性标志物可用于其分离及鉴定.

关 键 词:表皮干细胞  细胞分离  鉴定
文章编号:0258-879X(2004)08-0890-03
收稿时间:2003-12-15
修稿时间:2003-12-15

Isolation and identification of epidermal stem cells
NIU Yun-Fei,LU Wei,XIA Zhao-Fan. Isolation and identification of epidermal stem cells[J]. Former Academic Journal of Second Military Medical University, 2004, 25(8): 0890-0891
Authors:NIU Yun-Fei  LU Wei  XIA Zhao-Fan
Abstract:Epidermal stem cells (ESCs) play a critical role in homeostasis and wound repair of skin tissue. Since ESCs are rare (fewer than 10% in total basal cells population) and lack specific markers,it is difficult to isolate and identify them from keratinocytes. Currently ,isolation of ESCs was achieved mainly by fast adhesion of ESCs to extracelluar matrix or flow cytometry. Several specific markers have been found in recent years for the identification of ESCs.
Keywords:epidermal stem cells  cell separation  identification
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