基于~1H NMR代谢组学技术探讨党参不同炮制品对免疫抑制大鼠的影响

目的基于核磁共振(1H NMR)代谢组学技术结合多元统计分析,研究环磷酰胺(CTX)所致免疫抑制大鼠血清中内源性代谢产物的变化规律,筛选党参、米炒党参、蜜炙党参提高免疫力的最佳炮制品,并探讨其作用机制。方法采用腹腔注射CTX复制免疫抑制模型,给药最后1 d收集大鼠血液,进行免疫细胞、免疫因子检测以及1H NMR代谢组学测试。结果党参及其炮制品均可提高免疫低下大鼠血清中白细胞、B淋巴细胞数及T细胞CD4+/CD8+值;党参、蜜炙党参调节IL-2、IgG、sIgA的效果优于米炒党参;蜜炙党参可明显改善免疫低下大鼠血清中亮氨酸、异亮氨酸、丙氨酸、谷氨酸、谷氨酰胺、萄葡糖、缬氨酸等物质的含量。结论蜜炙党参改善CTX所致的免疫低下效果最好,主要通过3条代谢通路来调节,分别为缬氨酸、亮氨酸、异亮氨酸代谢,谷氨酸、谷氨酰胺代谢,以及丙氨酸、天冬氨酸、谷氨酸代谢。

Effects of different processed products of Codonopsis pilosula on immunosuppressive rats based on ~1H NMR metabolomics

Aim Based on 1H NMR metabonomics combined with multivariate statistical analysis, to study the changes of serum endogenous metabolites from immunosuppressive rats induced by cyclophosphamide(CTX), and to screen the best processed products of Codonopsis pilosula , rice-fried Codonopsis pilosula and honey-fried Codonopsis pilosula in improve immunity, and to explore its mechanisms. Methods CTX was administered intraperitoneally(i.p.) to induce the immunosuppressive model. Serum was collected by aorta abdominalis on the last day for detecting immunocyte, immunokine and 1H NMR analysis. Results Codonopsis pilosula and its processed products could increase the numbers of white blood cells and B lymphocytes, as well as the ratio of CD4 + and CD8 + T cells. The regulation effects of honey-fried Codonopsis pilosula and Codonopsis pilosula on IL-2, IgG and sIgA were better than those of rice-fried Codonopsis pilosula . Honey-fried Codonopsis pilosula could significantly improve the levels of leucine, isoleucine, alanine, glutamate, glutamine, glucose and valine etc. Conclusions Honey-fried Codonopsis pilosula could play the best role in immunosuppressive rats through regulating valine, leucine and isoleucine metabolism, glutamate and glutamine metabolism, alanine, aspartate and glutamate metabolism.