复方蜚蠊提取物PB对异体抗原联合醋酸诱导大鼠慢性溃疡性结肠炎的作用机制

目的研究复方蜚蠊提取物PB对慢性溃疡性结肠炎(UC)大鼠的作用,并初步探讨其作用机制。方法采用异体抗原联合醋酸建立慢性UC模型,正常组及模型组灌肠给予氯化钠溶液,其余各组分别给予柳氮磺胺吡啶(SASP)、肠炎宁和PB低、中、高剂量药物。评价疾病活动指数(DAI)和病理组织学评分(HS);测定脏器指数和结肠黏膜指数(CMDI);ELISA法检测血清中IL-4、IL-8、IL-17、INF-γ、CRP、IgG,以及结肠黏膜中TNF-α、MPO、EGF的含量。结果与正常组比较,模型组IL-8、IL-17、INF-γ、CRP、IgG、TNF-α、MPO含量均明显升高,IL-4、EGF含量明显降低(P<0.01,P<0.05);PB各剂量组DAI、CMDI、HS、IL-8、IL-17、INF-γ、CRP、IgG、TNF-α、MPO含量均明显降低,IL-4含量明显升高(P<0.01,P<0.05)。结论复方蜚蠊提取物PB灌肠给药后能够有效缓解异体抗原联合醋酸诱导的大鼠慢性UC,其作用机制可能与促进Th2细胞亚群活化,使倾向于Th1的Th1/Th2平衡向Th2移动,发挥细胞免疫和体液免疫调节作用,达到免疫平衡稳态有关。

Study on mechanism of action of Blattidae compound PB against chronic ulcerative colitis induced by alloantigen and acetic acid in rats

Aim To study the effect of Blattidae compound PB on chronic UC induced by alloantigen combined with acetic acid in rats, and to preliminarily explore its mechanism. Methods Chronic UC model was established using alloantigen combined with acetic acid. Normal group and model group were given sodium chloride solution by enema, while the other groups were given respectively to the SASP, the Changyanning and PB low, medium and high doses. The experiment was determined by evaluating DAI, CMDI, HS and the visceral index. Serum IL-4, IL-8, IL-17, CRP, IgG and colonic mucosa TNF- MPO EGF were detected by ELISA. Results Compared with normal group, the levels of IL-8, IL-17, INF-γ, CRP, IgG, TNF-α, MPO in model group significantly increased, while the levels of IL-4, EGF decreased significantly ( P <0.01, P <0.05). But the levels of DAI,CMDI, HS, IL-8, IL-17, INF-γ, CRP, IgG, TNF-α, MPO in each dose of PB groups were significantly lower than those of model group, and the levels of IL-4, EGF were significantly higher than those of model group ( P <0.01, P <0.05). Conclusions Blattidae compound PB can effectively relieve chronic ulcerative colitis induced by alloantigen combined with acetic acid in rats, and its mechanism may be related to promoting Th2 cell activation, changing Th1-prone Th1/Th2 balance to Th2, which regulated cell immunity and humoral immunity to achieve the steady state immune balance.