龟鹿二仙胶对创伤后应激障碍大鼠行为学和HPA轴功能的影响

目的 观察创伤后应激障碍(PTSD)大鼠下丘脑-垂体-肾上腺(HPA)轴功能和行为学的变化及龟鹿二仙胶对其的影响。方法 采用单一延长应激(SPS)的方法建立大鼠PTSD模型,采用旷场实验、高架十字迷宫实验,检测大鼠自主活动及焦虑水平;应用地塞米松抑制实验,检测大鼠HPA轴快速负反馈功能;采用ELISA法检测血浆皮质酮(CORT)水平和促肾上腺皮质激素(ACTH)含量。结果 PTSD模型大鼠d 8开始出现HPA轴快速负反馈功能增强,并持续到d22。模型组大鼠d 8焦虑水平升高,持续到d29。此外,d29还出现自主活动下降和血浆CORT水平升高。龟鹿二仙胶连续给药14 d后能明显抑制HPA轴负反馈功能的增强;连续给药21 d后能明显提高大鼠的自主活动能力,降低其焦虑水平和血浆CORT水平。结论 龟鹿二仙胶能够改善PTSD大鼠的行为学异常,其作用机制可能与调节HPA轴的功能紊乱有关。

Effect of Guiluerxianjiao on behaviors and HPA axis function in a rat model of post-traumatic stress disorder

Aim To investigate the effects of Guiluerxianjiao (GLEXJ) on the change of behaviors and HPA axis function in a rat model of post-traumatic stress disorder (PTSD).Methods Single-prolonged stress (SPS) was used to establish the model of PTSD.The level of locomotor activities and anxiety behaviors were assessed by open field test and elevated plus maze test.The hypothalamic-pituitary-adrenal fast negative feedback function was assessed by dexamethasone suppression test.The contents of both plasma CORT and ACTH were measured by ELISA.Results On day 8 after SPS, model group showed enhanced HPA fast negative feedback and continued to day22.The anxiety level of model group increased on day 8 and lasted on day29.While on day29, locomotor activities decreased and plasma CORT content increased.When continuously treated by GLEXJ for14 days, the enhanced HPA axis negative feedback function was significantly inhibited in GLEXJ group.After21 days of continuous administration, locomotor activities increased.In addition, the level of anxiety and CORT content were down-regulated in GLEXJ group.Conclusions GLEXJ could mitigate behavior disorder in a rat model of PTSD, which may be related to regulating HPA axis dysfunction.