基于脂质代谢组学评价五味子素B诱导的急性高甘油三酯血症小鼠模型

目的利用脂质代谢组学技术对五味子素B(schisandrin B,Sch B)诱导的小鼠高甘油三酯血症模型进行评价和提供新的实验依据。方法♂ICR小鼠分为4组:正常饮食(ND)组; ND+Sch B组;高脂高糖饮食(HFFD)组; HFFD+Sch B组。生化法检测血清甘油三酯(TG)和总胆固醇水平;利用超高效液相色谱-四极杆飞行时间质谱联用仪(UPLCQ-TOF/MS)的代谢组学技术方法测定各组小鼠血清中脂类代谢物的变化。结果 ND+Sch B组与ND组比,筛选出27个差异代谢物,分别为TG类18个、磷脂酰胆碱(PC) 7个、磷脂酰乙醇胺(PE) 2个; HFFD组与ND组比,筛选出27个差异代谢物,分别为神经鞘磷脂6个、PC 13个、胆甾醇酯(CE) 2个、TG类5个、磷脂酰肌醇1个; HFFD+Sch B组与HFFD组比,筛选出25个差异代谢物,分别为TG类14个、CE 1个、PC 6个、PE 4个。结论 Sch B诱导的高甘油三酯血症动物模型涉及血清脂质代谢组学的改变。

Evaluation of schisandrin B-induced mouse model of hypertriglyceridemia based on lipid metabolomics

Aim To evaluate the mouse model of hypertriglyceridemia(hTG) induced by schisandrin B(Sch B) using lipid metabolomics technology. Methods Male ICR mice weighing 23~27 g were randomly divided into four groups:① mice fed with normal diet(ND group);② mice fed with ND and treated with Sch B(ND+Sch B group);③ mice fed with high fat/fructose diet(HFFD group;fat, 10%;fructose, 10%;w/w),and ④ mice fed with HFFD and treated with Sch B(HFFD+Sch B group). Based on our previous research, Sch B at a single dose of 2 g·kg^-1 was orally administered to the animals in the current study. Forty-eight hours later, serum samples were obtained from the orbital vein. Serum triglyceride(TG) and total cholesterol(TC) were analyzed by biochemical method. The metabolic fingerprint spectrum of serum in all groups were obtained and analyzed using ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry(UPLC-Q/TOF-MS) method. The differences of metabolic spectra in every two groups were compared via the multivariate statistical methods. Results Compared with ND group, three kinds(27 markers) of differential metabolites were identified in ND+Sch B group, including 18 TG, 7 phosphatidylcholine(PC), and 2 phosphatidylethanolamine(PE). Compared with ND group, five kinds(27 markers) of differential metabolites were screened in HFFD group, including 6 sphingomyelin(SM), 13 PC, 2 cholesteryl ester(CE), 5 TG and 1 phosphatidylinositol(PI).Compared with HFFD group, four kinds(25 markers) of differential metabolites were found in HFFD+Sch B group, involving 14 TG, 1 CE, 6 PC and 4 PE. Conclusion These findings suggest that the animal model of hypertriglyceridemia established by Sch B involves the alteration of serum lipid metabolomics.