The Release of Histamine,Heparin and Granule Protein from Rat Mast Cells Treated with Compound 48/80 in vitro

Fillion , G. M. B., S. A. Slorach and B. Uvnäs . The release of histamine, heparin and granule protein from rat mast cells treated with compound 48/80 i n vitro. Acta physiol. scand. 1970. 78. 547–560. The quantitative relationship between the release of histamine, heparin and granule protein from rat mast cells exposed to compound 48/80 in vitro has been investigated in order to clarify the mechanism of action of this releaser. A correlation was found between the three release curves. The ratio of heparin to granule protein was similar in extruded granules and granules remaining in the cell after compound 48/80 treatment. From these results we conclude that the principal mechanism of histamine release induced by this agent involves an initial extrusion of histamine-containing granules, followed by an exchange of histamine in the granules for cations in the extracellular medium.