| MAPK/ERK信号通路在益气解毒方水提物诱导鼻咽癌细胞凋亡中的作用 |
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| 引用本文： | 胡晶,刘洁,徐冰雁,戴娜,胡梅,周芳亮,史红健,罗晶婧,蔺婷,何迎春.MAPK/ERK信号通路在益气解毒方水提物诱导鼻咽癌细胞凋亡中的作用[J].中国药理学通报,2019(11):1613-1621. |
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| 作者姓名： | 胡晶 刘洁 徐冰雁 戴娜 胡梅 周芳亮 史红健 罗晶婧 蔺婷 何迎春 |
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| 作者单位： | 1.湖南中医药大学研究生学院;2.湖南中医药大学中医药防治眼耳鼻咽喉疾病湖南省重点实验室;3.长沙医学院中医学院;4.湖南中医药大学医学院 |
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| 基金项目： | 国家自然科学基金资助项目(No 81573721,81874408);湖南省自然科学基金资助项目(No 2016JJ6117,2017JJ3246);湖南中医药大学研究生创新项目(No 2017CX33) |
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| 摘 要： | 目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响;Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果 益气解毒方水提物能抑制CNE1、CNE2细胞增殖( P <0.05)、诱导凋亡( P <0.05);药物作用48 h后,Survivin、XIAP、Bcl-2表达下降,Bax表达上升,MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK表达下降( P <0.05);在此基础上,加入激活剂ISO和益气解毒方水提物后,与单用益气解毒方水提物相比,p-c-Raf、p-MEK、p-ERK1/2表达上调,Survivin、XIAP、Bcl-2表达增加,Bax表达下降,促凋亡效应也降低( P <0.05)。结论 益气解毒方水提物可诱导鼻咽癌细胞凋亡,该效应与其抑制MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK1/2表达,进而下调Survivin、XIAP、Bcl-2表达,上调Bax表达有关。
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| 关 键 词： | 益气解毒方 鼻咽癌 细胞凋亡 MAPK/ERK信号通路 Survivin XIAP |
Role of MAPK/ERK signaling pathway in apoptosis of nasopharyngeal carcinoma cells by Yiqi Jiedu formula |
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| HU Jing,LIU Jie,XU Bing-yan,DAI Na,HU Mei,ZHOU Fang-liang,SHI Hong-jian,LUO Jing-jing,LIN Ting,HE Ying-chun.Role of MAPK/ERK signaling pathway in apoptosis of nasopharyngeal carcinoma cells by Yiqi Jiedu formula[J].Chinese Pharmacological Bulletin,2019(11):1613-1621. |
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| Authors: | HU Jing LIU Jie XU Bing-yan DAI Na HU Mei ZHOU Fang-liang SHI Hong-jian LUO Jing-jing LIN Ting HE Ying-chun |
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| Institution: | (Post-Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology andOtolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China;College of Medicine, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Provincial ResearchEngineering Technology Center for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases withTraditional Chinese Medicine and Visual Function Protection, Hunan University of Chinese Medicine, Changsha 410208, China;College of traditional Chinese Medicine, Changsha Medical University, Changsha 410219,China) |
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| Abstract: | HU Jing;LIU Jie;XU Bing-yan;DAI Na;HU Mei;ZHOU Fang-liang;SHI Hong-jian;LUO Jing-jing;LIN Ting;HE Ying-chun(Post-Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology andOtolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China;College of Medicine, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Provincial ResearchEngineering Technology Center for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases withTraditional Chinese Medicine and Visual Function Protection, Hunan University of Chinese Medicine, Changsha 410208, China;College of traditional Chinese Medicine, Changsha Medical University, Changsha 410219,China) |
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| Keywords: | Yiqi Jiedu formula nasopharyngeal carcinoma cell apoptosis MAPK/ERK signaling pathway Survivin XIAP |
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