酸敏感离子通道-1a在急性肺损伤大鼠肺组织中的表达及作用初探

目的 探讨酸敏感离子通道-1a(ASIC1a)在急性肺损伤(acute lung injury,ALI)大鼠肺组织中的表达及可能的作用。方法 SPF级♂SD大鼠32只,随机分为正常组、ALI组、阿米洛利组和阳性药物组。对动脉血进行血气分析,观察肺组织病理学,肺组织称重后计算湿干重比,ELISA检测血清中TNF-α的表达,qPCR和Western blot检测肺组织中ASIC1a的表达。结果 与正常组相比,模型组肺组织损伤明显;肺组织湿干重比明显升高;TNF-α的表达水平明显升高(P<0.01)。阿米洛利组及阳性药物组肺组织损伤明显减轻;肺组织湿干重比较模型组明显下降,TNF-α的表达水平明显降低(P<0.01)。免疫组化、qPCR和Western blot结果显示,与正常组相比,模型组ASIC1a的表达量明显升高(P<0.01),阿米洛利组与模型组相比,ASIC1a的表达量明显减少(P<0.01)。结论 在脂多糖诱导的ALI大鼠肺组织中ASIC1a的表达增加,其可能参与ALI的发生、发展。

Expression and role of acid-sensitive ion channel-1a in lung tissues of rats with acute lung injury

Aim To explore the expression and possible effect of acid sensing ion channel-1a(ASIC1a) in lung tissues of rats with acute lung injury(ALI).Methods Thirty-two male Sprague-Dawley(SD) rats were randomly divided into four groups:control group,model group,amiloride group and Dex group.Blood gas analysis of arterial blood,observation of lung histopathology were performed and wet/dry ratio was calculated after weighing of lung tissues,then the expression of TNF-α in serum was detected by ELISA,and the expression of ASIC1a in lung tissues was also detected by qPCR and Western blot.Results Compared with control group,the lung tissue damage was obvious in model group,the wet/dry ratio of lung tissues obviously increased,and the expression level of TNF-α markedly increased (P<0.01).Lung tissue injury was significantly alleviated in amiloride group and Dex group,the wet/dry ratio of lung tissues obviously decreased,and the expression level of TNF-α significantly decreased (P<0.01).Immunohistochemistry,qPCR and Western blot results showed that,compared with control group,the expression of ASIC1a significantly increased in model group (P<0.01),and markedly decreased in amiloride group (P<0.01).Conclusions The expression of ASIC1a increases in acute lung injury induced by lipopolysaccharide,which may be involved in the occurrence and development of ALI.