小分子阿胶抗疲劳、抗氧化及止血作用研究

目的探讨小分子阿胶的抗疲劳、抗氧化及止血作用。方法建立大鼠复合血虚模型,检测游泳力竭时间、血液学指标及血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(maleic dialdehyde,MDA)、脂质过氧化物(lipid peroxide,LPO)水平,考察小分子阿胶的抗疲劳、抗氧化作用。测定ICR小鼠出血时间(bleeding time,BT)、凝血时间(clotting time,CT)。建立大鼠血热出血模型和肝素化出血模型,考察小分子阿胶在止血方面的作用及可能的机制。结果与模型组比较,小分子阿胶各剂量均明显延长大鼠游泳力竭时间(P<0.05,P<0.01),明显降低血清MDA、LPO含量(P<0.05,P<0.01);小分子阿胶1.500、0.375 g·kg^-1明显升高大鼠血中淋巴细胞数量(P<0.05);3.00、1.50 g·kg^-1明显缩短小鼠BT和CT(P<0.05);1.500 g·kg^-1逆转延长的凝血酶原时间(P<0.05,P<0.01),逆转血细胞的不良改变。结论小分子阿胶具有抗疲劳、抗氧化、增强耐力的作用,并有止血的功效。

Study on anti-fatigue,anti-oxidative and hemostatic effects of small molecule Asini Corii Colla

Aim To study the anti-fatigue,anti-oxidative and hemostatic effects of small molecule Asini Corii Colla(SMACC). Methods Rat model of complex blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismu-tase(SOD),serum maleic dialdehyde(MDA), and blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismutase( SOD),serum maleic dialdehyde( MDA),and lipid peroxide( LPO) levels,aiming to explore the anti-fatigue and anti-oxidative effects of SMACC. ICR mice were used to measure the blood clotting time( CT) and bleeding time( BT). Fevered and bleeding model and the heparinized bleeding model in rats were established to investigate the effect of SMACC in hemostasis and its possible mechanism. Results Compared with model group,SMACC significantly prolonged the swimming time of model rats(P<0.05,P<0.01)and decreased the content of serum MDA,LPO( P<0. 05,P<0.01) at doses of 1. 500,0. 750,0. 375 g·kg-1,and increased the number of lymphocytes in the blood at doses of 1. 500,0. 375 g·kg-1( P<0.05).SMACC of 3. 00,1. 50 g · kg-1 significantly reduced the BT and CT( P<0.05). SMACC markedly reversed the prolonged prothrombin time( P<0.05) and the adverse changes of the hematological indicators.Conclusions SMACC has the pharmacological effects of anti-fatigue,anti-oxidation and enhancing endurance,and also the effects of hemostasis and convergence.