人参皂甙Rd对匹罗卡品致癎大鼠模型中netrin-1表达及神经元凋亡的影响 |
| |
引用本文: | 郑佳丽,杨金升,刘学娟,马亚杰. 人参皂甙Rd对匹罗卡品致癎大鼠模型中netrin-1表达及神经元凋亡的影响[J]. 中华老年心脑血管病杂志, 2011, 13(5). DOI: 10.3969/j.issn.1009-0126.2011.05.024 |
| |
作者姓名: | 郑佳丽 杨金升 刘学娟 马亚杰 |
| |
作者单位: | 1. 兰州大学第二临床医学院,730000 2. 兰州军区兰州总医院神经内科 |
| |
摘 要: | 目的探讨人参皂甙Rd(GSRd)对大鼠癫癎持续状态后齿状回netrin-1表达及海马神经元凋亡的影响。方法健康Wistar大鼠30只,随机分为颞叶癫癎组、GSRd组和对照组,每组10只。氯化锂-匹罗卡品建立大鼠颞叶癫癎模型,采用免疫组织化学法及TUNEL法观察颞叶癫癎组、GSRd组和对照组齿状回netrin-1蛋白表达及海马神经元凋亡细胞数情况。结果与对照组比较,颞叶癫癎组大鼠30 d齿状回netrin 1蛋白表达明显增高,海马CA3区TUNEL阳性细胞数在癫癎持续状态后7 d明显增高,差异有统计学意义(P<0.05);与颞叶癫癎组比较,GSRd组大鼠30 d齿状回nctrin l蛋白表达明显降低,海马CA3区TUNEL阳性细胞数在癫癎持续状态后7 d明显降低,差异有统计学意义(P<0.05)。结论 GSRd可能通过下调netrin 1的表达,并使神经元凋亡数目减少,从而发挥对神经元的保护作用。
|
关 键 词: | 人参皂甙类 癫痫 神经元 海马 细胞凋亡 氯化锂 模型 动物 |
Effects of ginsenoside-Rd on expression levels of netrin-1 and neuron apoptosis in epilepsy model |
| |
Abstract: | Objective To investigate the effects of ginsenoside-Rd(GSRd) on the expression levels of netrin-1 and neuron apoptosis in the rat hippocampus after lithium-pilocarpine induced epilepsy. Methods Thirty Wistar rats were randomly divided into the NS control group,temporal lobe epilepsy(TLE) group and GSRd group.The TLE animal model was established using lithium-pilocarpine. Netrin-1 proteins were detected with immunohistochemical method.The neuron apoptosis was observed with TdT-mediated dUTP nick end labeling(TUNEL) method.Results The expression of netrin-1 in GSRd group was notably lower than that of TLE group(P<0.05).The TUNEL positive cells in hippocampus CA3 of TLE group were more than those of NS control group(P<0.05).TUNEL positive cells in TLE group were significantly more than those in NS control group(P<0.05).TUNEL positive cells in GSRd treated group significantly decreased as compared with TLE group(P<0.05).Conclusions GSRd may serve as an effective agent for curing the brain damage after TLE in vivo.The mechanism of protecting the neuron may include downregulating the expression of netrin-1 and reducing the number of apoptotic neurons. |
| |
Keywords: | ginsenosides epilepsy neurons hippocampus apoptosis lithium chloride models,animal |
本文献已被 万方数据 等数据库收录! |
|