补阳还五汤提取液对Schwann细胞氧化损伤的保护作用

本实验建立了Schwann细胞的体外氧化损伤模型,从对Schwann细胞的抗氧化损伤作用探讨补阳还五汤的疗效机制。用原代培养的Schwann细胞建立氧化损伤模型,将培养细胞分为氧化损伤组,补阳还五汤处理组及正常组:(1)应用MTT方法检测细胞的活性;(2)应用生化技术检测细胞内超氧化物歧化酶(SOD)的含量;(3)采用荧光探针Fluo-3-AM标记细胞,用激光共聚焦显微镜观察H_2O_2对Schwann细胞内游离钙([Ca2+])抗氧化损伤的影响。结果显示:与对照组相比,H2O2处理组细胞活性降低(P<0.01),SOD含量明显减少(P<0.01),而补阳还五汤预处理后细胞内[Ca2+]i升高明显被减弱(P<0.01),细胞存活率明显升高(P<0.01)。以上结果表明补阳还五汤能通过抗过氧化损伤保护Schwann细胞。

THE PROTECTIVE EFFECTS OF BUYANGHUANWU DECOCTION ON THE OXIDATIVE INJURIED SCHWANN CELL

To investigate the protective effects of Buyanghuanwu Decoction on the oxidative injuried Schwann cell;after making the oxidative injuried model of the cultured Schwann cell: (1) MTT was used to observe cell activity of the Schwann cell treated with hydrogen peroxide; (2) Detecting the intracellular superoxide dismutase (SOD); (3) The fluorescent probe Fluo-3-AM was used to detect the intracellular Ca~ 2 levels by using Lasers Scanning Confocal Microscopy (LSCM). The results showed that, compared with the control group,the oxidative injured Schwann cells had decreased cell activity and elevated intracellular Ca~ 2 level . However, these changes were significantly inversed by Buyanghuanwu Decoction preconditioned group. These results suggest that Buyanghuanwu Decoction has good antioxidated effect to protect the Schwann cells.