黏萼蝇子草蜕皮甾酮类成分的分离与鉴定

目的对黏萼蝇子草根的化学成分进行研究。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱和反相半微量制备型高效液相色谱分离纯化,根据ESI-MS、1H-NMR和13C-NMR等谱学数据进行结构鉴定。结果从黏萼蝇子草根的乙醇提取物分离得到5个蜕皮甾酮类化合物,分别鉴定为20-羟基蜕皮激素(20-hydroxyecdysone,1)、1-epi-integristerone A(2)、abutasterone(3)、旌节花甾酮A(stachysterone A,4)和15-羟基旌节花甾酮A(15-hydroxystachysterone A,5);另外得到胡萝卜苷(daucosterol,6)和β-谷甾醇(β-sitosterol,7)2个甾醇类化合物。结论化合物2-5为蝇子草属植物中首次分离的化合物。

Isolation and identification of ecdysterones from Silene viscidula Franch.

Objective To study the constituents of the extract from the root of Silene viscidula Franch. Methods The chemical constituents were isolated by silica gel column, open ODS column, Sephadex LH-20 column chromatographies, and semi-preparative HPLC. Chemical characters and spectroscopic analysis were employed for the structural identification. Results Five ecdysterone compounds were obtained and their structures were identified as 20-hydroxyecdysone (1), 1-epi-integristerone A (2), abutasterone (3), stachysterone (4) and 15-hydroxystachysterone A (5), along with two sterol compounds daucosterol (6) and b-sitosterol (7). Conclusions Compounds 2-5 are obtained from the genus Silene for the first time.