非共价涂层毛细管电泳法测定黄连及其炮制品中3种生物碱的含量

目的建立同时测定黄连药材中小檗碱、巴马汀和药根碱含量的毛细管电泳方法。方法采用聚凝胺-聚丙乙烯磺酸钠涂层毛细管柱(33 cm×50μm I.D.,有效长度为24.7 cm),运行缓冲液为500 mmol.L-1Tris-甲醇(体积比为70∶30)缓冲溶液(8 mol.L-1,磷酸调节pH 7.5),分离电压为22 kV,检测波长为230 nm,温度为20℃,压力自动进样(5 000 Pa×6 s),阴极检测。结果在所选电泳条件下,黄连中的生物碱组分在4.5 m in内得到良好分离,盐酸小檗碱、盐酸巴马汀和盐酸药根碱的线性范围分别为199.8~1 998、48.45~484.5和47.3~473 mg.L-1,线性关系良好,加样回收率分别为102.8%、100.2%和97.7%。结论该方法可应用于黄连药材中生物碱的分析测定。

Determination of three alkaloids in Coptidis Rhizoma by noncovalently bilayer-coated capillary electrophoresis

Objective To develop a capillary electrophoretic method with a noncovalently coated capillary for simultaneous determination of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride in Coptidis Rhizoma.Methods A PB-PSS coated capillary(33 cm × 50 μm I.D.,effective length is 24.7 cm) was prepared.Separation was carried out at 22 kV in 500 mmol·L-1 Tris(pH 7.5)-CH3OH(V∶V=70∶30) buffer.The temperature was kept at 20 ℃.The detection wavelength was set at 230 nm.Pressure injection of 5 000 Pa× 6 s was employed.Results The linear ranges of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride were 199.8-1 998,48.45-484.5 and 47.3～473 mg·L-1,respectively.The average recoveries of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride were 102.8%,100.2% and 97.7%,respectively.Conclusions The method is can be applied for the determination of alkaloids in Coptidis Rhizoma.