| 普罗布考抑制氧化低密度脂蛋白上调巨噬细胞清道夫受体CD36的表达 |
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| 引用本文： | 张社兵,姜德谦,欧柏清,刘虹,张小炼,田轶伦.普罗布考抑制氧化低密度脂蛋白上调巨噬细胞清道夫受体CD36的表达[J].高血压杂志,2008,16(1):55-59. |
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| 作者姓名： | 张社兵 姜德谦 欧柏清 刘虹 张小炼 田轶伦 |
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| 作者单位： | 中南大学湘雅二医院心血管内科,湖南长沙410011 |
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| 摘 要： | 背景普罗布考是一降脂药，同时具有抗氧化特性，近年来的研究表明其可以通过多种机制抑制动脉粥样硬化的形成，降低经皮冠状动脉成形术后再狭窄的发生。目的探讨普罗布考对氧化低密度脂蛋白（Ox-LDL）刺激巨噬细胞后清道夫受体CD36表达的抑制效应。方法用不同浓度的Ox-LDL刺激人单核细胞系THP-1源性巨噬细胞及用不同浓度普罗布考和核因子κBp65（NF-κBp65）特异性抑制剂（PDTC）预处理细胞后再用氧化低密度脂蛋白刺激，半定量RT—PCR方法检测CD36和NF-κBp65的mRNA的表达，Western-blot检测CD36和细胞核NF-κBp65蛋白表达水平。结果THP-1单核细胞分化成巨噬细胞后，CD36和NF-κBp65 mRNA和蛋白的表达上调；不同浓度的Ox-LDL刺激后，CD36mRNA和蛋白的表达呈浓度依赖性增加，同时伴NF-κBp65 mRNA和蛋白的表达增加；PDTC和普罗布考干预后，NF-κBp65和CD36的mRNA和蛋白表达下调。结论Ox-LDL可以通过NF-κBp65途径上调巨噬细胞CD36的表达；而普罗布考则可以抑制CD36的表达，这种作用与抑制NF-κBp65有关。
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| 关 键 词： | 清道夫受体CD36 核因子κBp65 普罗布考 氧化低密度脂蛋白 巨噬细胞 |
Probucol Inhibits Expression of CD36 Induced by Oxidized Low-density Lipoprotein in Macrophages |
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| ZHANG She-bing JIANG De-qian,OU Bai-qing,LIU Hong,ZHANG Xiao-lian,TIAN Yi-lun.Probucol Inhibits Expression of CD36 Induced by Oxidized Low-density Lipoprotein in Macrophages[J].Chinese Journal of Hypertension,2008,16(1):55-59. |
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| Authors: | ZHANG She-bing JIANG De-qian OU Bai-qing LIU Hong ZHANG Xiao-lian TIAN Yi-lun |
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| Institution: | ZHANG She-bing JIANG De-qian,OU Bai-qing,LIU Hong,ZHANG Xiao-lian,TIAN Yi-lun.Department of Cardiology,The Second Xiangya Hospitol of Central South University,Changsha 410011,China |
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| Abstract: | Background Probucol is an anti-hyperlipidemic agent with strong anti-oxidative property.Acumulated evidences demonstrate that it can inhibit atherogenesis and restenosis after percutaneous transluminal coronary angioplasty by multi-mechanism.Objective To investigate the expression of CD36 induced by Ox-LDL and to observe the inhibitory effect of probucol in THP-1 derived macrophages.Methods THP-1 derived macrophages were incubated with Ox-LDL and were pretreated with different concentration of probucol and ... |
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