水溶性大豆异黄酮对破骨细胞分化和功能的影响

目的:研究水溶性大豆异黄酮(WSSI)对1,25-二羟基维生素D3诱导兔骨髓细胞分化形成破骨细胞样细胞以及兔成熟破骨细胞骨吸收功能的影响。方法:通过TRAP染色对骨髓细胞诱导分化形成的TRAP阳性多核巨细胞计数;用显微摄影结合计算机图像分析测定骨吸收造成的陷窝数目及表面积,以评价破骨细胞活性;用扫描电镜观察骨吸收陷窝的形态。结果:浓度为20.0、4.0、2.0和0.4μg/ml的水溶性大豆异黄酮既能抑制破骨细胞样细胞的形成(P<0.001),还能抑制成熟破骨细胞的骨吸收功能(P<0.001),具体表现在随着浓度的升高骨吸收陷窝数目及表面积减少。结论:WSSI可以明显抑制破骨细胞样细胞的形成和成熟破骨细胞的骨吸收功能。

EFFECTS OF WATER-SOLUBLE SOYBEAN ISOFLAVONES ON DIFFERENTIATION AND FUNCTION OF OSTEOCLASTS

Objective: To study the effects of water-soluble soybean isoflavones (SI) on formation of osteoclast-like cells in bone marrow induced by 1,25-dihydroxyvitamin D3 and bone-resorbing activity of rabbit mature osteoclasts in rabbits. Method: The number of tartrate resistant-acid phosphatase-positive TRAP(+)] multinucleated cells were counted by light microscope. The number and surface area of bone resorption pits were measured by photomicrography and image analysis to evalute the bone-resorbing activity of rabbit mature osteoclasts. The pit morphology was observed under scanning electron microscope. Results: Water-soluble SI inhibited the formation of osteoclast-like cells at the concentrations of 20.0, 4.0, 2.0 and 0.4 μg/ml (P<0.001). It also inhibited osteoclastic activity as indicated by reduction in the numbers and surface areas of the pits (P<0.001). Conclusion: Water-soluble SI can significantly inhibit the formation of osteoclast-like cells and bone resorption function of rabbit mature osteoclasts.