吉林省地区朝鲜族和汉族健康人群醛固酮合酶基因（CYP11B2）-344T/C多态性分布的研究

目的分析我国吉林省地区朝鲜族和汉族健康人群醛固酮合酶基因（CYP11B2）-344T/C多态性的分布。方法应用聚合酶链反应（Polymerase Chain Reaction,PCR）和限制性片段长度多态性（Restriction Fragment LengthPolymorphism,RFLP）技术,对67名朝鲜族和59名汉族健康人群CYP11B2基因多态性进行检测,并比较基因型和等位基因频率的分布特征。结果 67名朝鲜族中CYP11B2基因CC、TC和TT基因型频率分别为9%（6/67）、22%（15/67）和69%（46/67）;59名汉族中CC、TC和TT基因型频率分别为19%（11/59）、27%（16/59）和54%（32/59）,两者差异无显著性（χ2=3.522;P=0.172）。朝鲜族人群C和T等位基因频率分别为20%和80%;汉族人群分别为32%和68%,两者差异具有显著性（χ2=4.763;P=0.029）。结论吉林省地区朝鲜族和汉族健康人群（CYP11B2）-344T/C基因型分布频率差异无显著性,朝鲜族健康人群T等位基因频率高于汉族。

Study on (CYP11B2)-344T/C gene polymorphism in chinese korea and chinese han healthy people in jilin province

Objective To investigate the distribution of （CYPllB2）-344T/C gene polymorphism of chinese han and chinese korea healthy people in jilin province. Methods The techniques of PCR and RFLP were used to detect the poly- morphism of CYPllB2 gene in 67 Korea and 59 Han healthy people. Results The frequency of CC,TC and TT is 9%, 22% and 69%,respected in Korea,and the frequency of CC,TC and TT is 19%,27% and 54% in Han. There were no remarkable differences of the frequency of genotype of CYPllB2 gene（P= 0. 172）between Korea and Han. But there was a significant difference in the frequency distribution of CYPllB2 alleles （P=0. 029） between Korea and Han. Con- clusion There were no remarkable differences of the frequency of genotype of CYPllB2-gene between korea and han. There was a significant difference in the frequency distribution of CYPllB2 alleles between korea and ban. Frequency distribution of T allele of korea is higher than that of han.