c-Met蛋白、端粒酶在肝细胞癌中的表达及临床意义

目的研究c-Met蛋白和端粒酶（Telomerase）在肝细胞癌中的表达,探讨二者在肝细胞癌发生发展中的作用。方法应用免疫组化（SP）对42例肝细胞癌组织,和10例肝细胞癌癌旁组织中的c-Met蛋白和端粒酶进行定量的检测。结果①c-Met和hTERT在肝细胞癌组织中的表达阳性率分别为76.19%和88.09%,对照组分别为30%和40%,两组间阳性率比较差异均有统计学意义（P〈0.05）;②c-Met和hTERT在肝细胞癌中表达两者之间无相关性（r=0.140,P〉0.05）;③c-Met的表达与性别、年龄、有无包膜、肝硬化及HBsAg无关（P〉0.05）,与肿瘤的转移有关（P〈0.05）;hTERT与性别、年龄、有无包膜、肿瘤的转移及HBsAg均无关（P〉0.05）,与肝硬化有关（P〈0.05）;④hTERT阳性肝细胞癌组织中c-Met（＋＋＋）、（＋＋）、（＋）和（-）分别为15、9、5和8例。hTERT阴性肝细胞癌组织中c-Met（＋＋＋）、（＋＋）、（＋）和（-）分别为0、2、1和2例,两组间比较有统计学差异（P〈0.05）。结论 c-Met蛋白和端粒酶在肝细胞癌的发生、发展和转移过程中起着重要的作用,与预后存在着密切关系;端粒酶阳性的肝细胞癌的侵袭转移能力要明显的强于端粒酶阴性的肝细胞癌组织,对二者定量的检测能给临床的治疗及预后带来一定的指导意义。

Expressions of c-Met and telomerase in hepatocellularcarcinoma and their clinical significances

Objective To study the expressions of c-Met and Telomerase in hepatocellular carcinoma and discuss the effects of c-Met and Telomerase in the occurrence and development of hepatocellular carcinoma. Methods The expres- sions of c-Met and Telomerase were examined using immunohis- tochemical SP staining method in 42 cases of hepato- cellular carcinoma and 10 cases of paracaneerous tissues. Results OThe positive rates of c-Met and hTERT expres- siong in hepatoeellular carcinoma tissues were 76.19% and 88.09% respectively, in paracaneerous tissues were g0~~ and 40o/40, there were significant differences between two groups（P~0. 05） ~（~）The expressions of c-Met and Telomer- ase in hepatocellular carcinoma have no correlation（r= 0. 140, P~0.05）.. （g）The expression of c-Met was correlated with extends of tumor（P~0.05） ; wasn＇t eorrelated with sex, age, Whether the envelope, Cirrhosis of Liver and HBsAg （P〉0.05） ;The expression of hTERT wasn＇t correlated with extends of tumor, sex,age, Whether the envelope and H B- sAg （P〉0.05） ,was correlated with Cirrhosis of Liver（p〈0.05） ;（4）The expressions of c-Met in hepatocellular carci- noma of hTERT positive were fifteen eases of （＋ ） ,nine cases of （＋＋） ,five case of （＋） ,eight case of（-）. The expressions of c-Met in hepatoeellular carcinoma of hTERT negative were zero cases of （＋＋＋ ）, two cases of （ ＋＋）, one ease of （＋）, two case of （-）. there were statistically significant differences between the two group（P〈0.05）. Conclu- sion c-Met and Telomerase play an important role in the occurrence development and the metastasis of hepatocellular carcinoma and have close .relationship with its prognosis,The abilities of metastasis and invasion in telomerase positive hepatocellular carcinoma are stronger than telomerase negative; Quantitative detection of c-Met and Telomerase may be bring some significance to-the clinical treatment and prognosis.