低水平乙型肝炎病毒表面抗原S区序列分析

目的对低水平乙型肝炎病毒表面抗原进行病毒S区基因分析。方法利用套式PCR法（Nested PCR）特异性扩增乙型肝炎病毒DNA的S区,对PCR扩增产物直接进行DNA序列分析,测序结果经Genotyping软件分型并与美国GeneBank中基因序列进行BLAST比对。结果 37例低水平乙型肝炎病毒表面抗原标本中,成功扩增并测序32份,其余5份扩增产物因不满足测序要求无法进行S区序列分析,32份成功测序标本经Genotyping分型,B型20例,C型12例,分别与GeneBank中收录的AF100309、AB014381同源性最高;血清学分型18例为adr,10例为adw,3例为ayw,1例为ayr,并发现21例S区核酸序列发生点突变,其中15例突变不会引起编码抗原决定簇氨基酸突变,为无义突变,而其他6例点突变均可引起抗原决定族编码的氨基酸的改变,从而导致表面抗原结构改变。结论对低水平乙型肝炎病毒表面抗原患者S区序列研究将为低水平乙型肝炎发病机制、流行病学以及个体化治疗奠定理论基础。

Analysis of Low levels of hepatitis B virus surface antigen S region sequence

Objective To analysis low levels of hepatitis B virus surface antigen virus S gene.Methods Using nested PCR（Nested PCR） and specificity amplification of hepatitis B virus DNA S area,PCR amplification products direct DNA sequence analysis,DNA sequencing results by Genotyping software typing and the United States GeneBank gene sequences of BLAST ratio.Results 37 cases of low levels of hepatitis B virus surface antigen in specimens,successful amplification and sequencing of 32,the remaining 5 amplification product does not meet the requirements for sequencing to S region sequence analysis,32 successful sequencing specimens by Genotyping typing,type B in 20 cases,12 cases of type C,and GeneBank published in AF100309、AB014381 homologous highest;serum type ADR in 18 cases,10 cases were ADW,3 cases were ayw,1 cases were Ayr,and found 21 cases of S nucleic acid sequence point mutations,including 15 cases of mutation does not cause encoding antigenic determinants for amino acid mutations,nonsense mutations,while the other 6 cases with point mutation may cause epitopes encoded amino acid changes,thereby causing the surface antigen structure change.Conclusion For low levels of hepatitis B virus surface antigen in patients with S region sequence research for low level hepatitis Mechanism,epidemiology and individualized treatment to lay the theoretical foundation.