管花马兜铃导致大鼠急性肾损伤的实验研究

目的观察管花马兜铃对大鼠急性肾损伤的作用. 方法用管花马兜铃水煎剂(15g*kg-1*d-1)对大鼠灌胃,建立大鼠急性肾损伤模型,检测有关肾功能指标,进行组织形态学观察.结果大鼠肾脏的主要功能改变为多尿、氮质血症、蛋白尿及血尿.组织形态学改变主要表现为急性肾小管变性、坏死.结论实验剂量的管花马兜铃可导致大鼠急性肾功能衰竭.

Experimental study of acute nephrotoxicity induced by Aristolochia Tubiflora. Dunn(ATD,a Chinese herb) in rats

Objective:To observe the acute renal injury induced by Aristolochia in rat.Methods:Experimental model of acute renal injury was established in the Sprague-Dawley rats with oral administration of decoction of ATD in a dosage of 15g/kg body weight/day for 8 consecutive days. Serum creatinine and urea nitrogen,urinary protein,urinary volume and hematuria were assayed. Renal histological examination was also performed after renal injury.Results:The major renal function changes of nephrotoxicity due to ATD included polyuria,azotemia,proteinuria and hematuria. Histological changes in the kidneys of the rats showed evidences of acute tubular necrosis.Conclusion:Acute renal tubular necrosis occurs in the Sprague-Dawley rats after oral administration of ATD in a dosage of 15g/kg body weight/day for 8 consecutive days. This Chinese herb is thought to be nephrotoxic and should be carefully used in medical practice.