骨科假体相关性感染的末端限制性片段长度多态性分析

背景：随着骨科假体植入的增加,假体相关性感染日益增多。只有深入研究感染中病原菌的分布特点,才能对假体相关性感染的机理和治疗方式有更全面的认识。目的：应用末端限制性片段长度多态性（T-RFLP）技术分析骨科假体相关性感染的细菌学特征。方法：选取骨科假体相关性感染患者的感染灶样本15例和非假体相关性感染患者的感染灶样本10例,提取样本总DNA,对其16S rDNA进行PCR扩增,应用T-RFLP技术分析样本中微生物群落分布情况。结果：假体相关性感染组检测阳性率为46.7%,非假体相关性感染组检测阳性率为20%;聚类分析结果显示相同解剖部位的感染中细菌群落分布均具有很高的相似性。结论：假体相关性感染检测阳性率高于非假体相关性感染;无论有无假体存在,相同解剖部位的感染中细菌群落的分布具有很高的相似性。

Analysis of orthopedic prosthesis-related infections with terminal restriction fragment length polymorphism

Background： With the increasing application of orthopedic prostheses implantation, the prosthesis-related infections are be- coming more and more common. Only in-depth study of the distribution characteristics of the pathogens in the infections can result in a comprehensive understanding of the mechanism and treatment of the prosthesis-related infections. Objective： To analyze the bacterial characteristics of the prosthesis- related infections with the Terminal Restriction Frag- ment Length Polymorphism （T-RFLP） technique. Methods： Select 15 cases of tissue samples from patients with orthopedic prosthesis-related infections and 10 cases of tissue samples from patients with orthopedic common infection, extract the total DNA of all the bacteria and amplify the 16S rDNA with PCR （Polymerase Chain Reaction） technology, then apply the T-RFLP technique to analyze the distribution of the bac- terium community. Results： The detected positive rates of the prosthesis-related infection group and the common infection group were 46.7% and 20% respectively. The result of cluster analysis showed that the distribution of bacterium communities had high similarity in the infections at the same anatomical position. Conclusions： The detected positive rate of the prosthesis-related infection is higher than that of the common infection. No matter there is protheses or not, infections at the same anatomical position has high similarity in the distribution of bacteri- um communities.