| 酶联免疫吸附试验在检测生殖器疱疹病毒抗原中的应用 |
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| 引用本文： | 刘文力,杨琳,金宁,张颖,李晓东.酶联免疫吸附试验在检测生殖器疱疹病毒抗原中的应用[J].中国中西医结合皮肤性病学杂志,2004,3(1):29-30. |
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| 作者姓名： | 刘文力 杨琳 金宁 张颖 李晓东 |
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| 作者单位： | 1. 沈阳市第七人民医院皮肤科,沈阳,110003
2. 沈阳市卫生监督所,沈阳,110014 |
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| 摘 要： | 目的探讨酶联免疫吸附试验(ELISA)检测泌尿生殖道分泌物及皮损中单纯疱疹病毒(HSV)抗原在临床中的应用价值.方法用聚合酶链反应(PCR)和ELISA方法检测泌尿生殖道及皮损中HSV DNA和HSV抗原,并对两种方法的检测结果进行比较.结果118例标本中,PCR检出41例阳性,ELISA 42例阳性.在118例标本中,111例两种方法结果相符,7例不相符合.PCR阳性的41例中,ELISA阳性38例(敏感性92.68%);PCR阴性的77例标本中,ELISA阳性4例(特异性94.81%).结论ELISA检测HSV抗原的方法可直接检测出泌尿生殖道及皮损中HSV病原体,从而为生殖器疱疹诊断提供准确的实验依据,其敏感性、特异性高,方便,快速,值得临床推广应用.
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| 关 键 词： | 生殖器疱疹 酶联免疫吸附试验 聚合酶链反应 |
| 文章编号： | 1672-0709(2004)01-0029-02 |
| 修稿时间： | 2003年11月4日 |
Application of Enzyme Linked Immunosorbent Assay in Detection of Genital Herpes Virus Antigen |
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| LIU Wen-li,YANG Lin,JIN Ning,ZHANG Ying,LI Xiao-dong.Application of Enzyme Linked Immunosorbent Assay in Detection of Genital Herpes Virus Antigen[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2004,3(1):29-30. |
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| Authors: | LIU Wen-li YANG Lin JIN Ning ZHANG Ying LI Xiao-dong |
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| Institution: | LIU Wen-li,YANG Lin,JIN Ning,ZHANG Ying,LI Xiao-dong
Department of Dermatology,The Seventh Hospital,Shenyang 110003,China |
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| Abstract: | Objectives To evaluate the use of enzyme linked immunosorbent assay (KLISA) method in the detection herpes simplex virus (HSV) antigen in genitourinary tract and dermal lesions herpes. Methods The specimens were tested with ELISA and polymerase chain reaction (PCR) in 118 patients. The results of 2 test methods was compared. Results Among 118 patients, HSV was detected in 42 by ELISA and 41 by PCR. 111 of 118 specimens showed the same results by both tests. In comparison with PCR, the relative sensitivities, specificities of ELISA were 92.68% and 94.81% respectively. Conclusion ELISA could directly detect HSV pathogen in genitourinary tract and dermal lesions and be simple, rapid, high sensitive and specific for the diagnosis of genital herpes. |
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| Keywords: | genital herpes enzyme linked immunosorhent assay polymerase chain reaction |
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