| 套式（Nested）PCR检测肺炎支原体与肺炎衣原体研究 |
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| 引用本文： | 糜祖煌,李迎伟.套式（Nested）PCR检测肺炎支原体与肺炎衣原体研究[J].中国优生与遗传杂志,1997,5(4):17-18. |
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| 作者姓名： | 糜祖煌 李迎伟 |
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| 作者单位： | 北京麦地卡优生遗传研究中心!101300(糜祖煌)，常州市第二人民医院儿科(李迎伟) |
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| 摘 要： | 本文报告根据肺炎支原体（Mp）和肺炎衣原体（Cpn）的16SrRNA基因序列，建立了套式（Nested）PCR检测方法。灵敏度试验表明，Mp套式PCR和Cpn套式PCR灵敏度分别高出Mp普通PCR和Cpn普通PCR二个数量极。
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| 关 键 词： | 肺炎支原体 肺炎衣原体 套式（Nested） PCR检测 |
Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae by the nested palymerase chain reaction |
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| Mi Zuhuang,et al..Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae by the nested palymerase chain reaction[J].Chinese Journal of Birth Health & Heredity,1997,5(4):17-18. |
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| Authors: | Mi Zuhuang |
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| Institution: | Mi Zuhuang, et al. |
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| Abstract: | The nested PCR was used the detect 16 S rRNA gene from Mycoplasma pneumoniae and Chlamydia pneumoniae. The sensitivity of the nested PCR amplification was higher than that of the common CPR (a one-step PCR). The nested PCR may be a specific, sensitive and simple method for Mycoplasma pneumoniae and Chlamydia pneumoniae. |
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| Keywords: | Mycoplasma pneumoniae Chlamydia pneumoniae Nested PCR |
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