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Similar structural requirements for T cell and antibody recognition of an MHC class I:peptide complex |
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| Authors: | Fugger Lars Andersen Peter Sejer Stryhn Anette Hansen Bjarke Svejgaard Arne Buus S[oslash]ren Engberg Jan |
| Affiliation: | a Dept. of Clinical Immunology, The State University Hospital, Dept. of Biology, The Royal Danish School of Pharmacy, Inst. of Microbiology and Immunology, University of Copenhagen Copenhagen Denmark |
| Abstract: | We have generated a monoclonal antibody (Fab 13.4.1) using the phage display technique, which recognizes the murine MHC class I molecule, Kk, in complex with the influenza virus derived peptide, HA255-262, but not in complex with other peptides. Using BIAcore analysis the kinetics of the antibody was used to calculate the KD to be about 10−9 M, which is typical of an antibody, and several orders of magnitude higher than the corresponding value for T cell receptors. Given the specificity and affinity of Fab13.4.1 we expected it to inhibit Kk restricted and HA255-262 specific T cell hybridomas, but not Kk restricted T cells with different specificity, which indeed was found to be the case. Thus, Fab13.4.1, recognizes a Kk:HA255-262 dependent epitope which is related to the epitope recognized by the specific T cells. A more detailed analysis of the structural requirements for T cell and antibody recognition of the Kk:HA255-262 complex revealed that they use similar contacts points to interact with this MHC class I:peptide complex. |
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