| 大鼠海马神经元模拟缺血时谷氨酸诱发电流改变 |
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| 引用本文： | 严晓晴,邓小明,孙继虎,徐美英,王春安,刘树孝. 大鼠海马神经元模拟缺血时谷氨酸诱发电流改变[J]. 中国组织工程研究与临床康复, 2003, 7(28): 3824-3825 |
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| 作者姓名： | 严晓晴 邓小明 孙继虎 徐美英 王春安 刘树孝 |
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| 作者单位： | 1. 解放军第二军医大学长海医院麻醉科,上海市,200433
2. 解放军第二军医大学生理学教研室,上海市,200433 |
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| 摘 要： | 目的:观察大鼠海马神经元模拟缺血时谷氨酸诱发电流的改变,探讨脑缺血神经元损伤的兴奋毒性机制,为中枢神经损伤的康复提供理论依据。方法:以原代培养的大鼠海马神经元为标本,采用全细胞膜片钳方法观察原代培养大鼠海马神经元模拟缺血时谷氨酸诱发电流改变。结果:当钳制电压为-60mV时,100μmol/L的N-甲基-D-天门冬氨酸(NMDA)、α-氨基-3羟基-5-甲基-4-异恶唑丙酸(AMPA)分别诱发一内向电流(INMDA,IAMPA),模拟缺血处理后的神经元INMDA、IAMPA明显增大。结论:升高的兴奋性氨基酸激活突触后膜的兴奋性氨基酸受体后引起神经细胞损伤。
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| 关 键 词： | 海马 神经元 谷氨酸 细胞学技术 |
| 文章编号： | 1671-5926(2003)28-3824-02 |
| 修稿时间： | 2003-02-01 |
Effects of simulated ischemia on glutamate-induced currents of hippocampal neurons in rats |
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| Xiao-Qing Yan,Xiao-Ming Deng,Mei-Ying Xu,Shu-Xiao Liu. Effects of simulated ischemia on glutamate-induced currents of hippocampal neurons in rats[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2003, 7(28): 3824-3825 |
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| Authors: | Xiao-Qing Yan Xiao-Ming Deng Mei-Ying Xu Shu-Xiao Liu |
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| Affiliation: | Xiao-Qing Yan,Xiao-Ming Deng,Mei-Ying Xu,Shu-Xiao Liu,Department of Anesthesiology,Changhai Hospital,Second Military Medical Universit y,Shanghai 200433,China Ji-Hu Sun,Chun-An Wang,Department of Physiology,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | AIM:To observe the change of simulated is chemia on glutamate-induced currents,and to explore the mechani sm of exitotoxicity of neurons-inju red in cerebral ischemia so as to provide th eory of of central injury of nerve reh a-bilitation.METHODS:The simulated ischemia on glutamate-induced currents in hippocampal primary-curtured neur ons from rats were observed by whole-cell clamp technique.RESULTS:Glutamate,N-methyl-D-aspartate(NMDA)and Alpha-amino-3-hydroxy-5-methylisoxazole-4-pro prionic acid(AMPA)induced an inward current at a holding potential(Vh)of-60mV by whole-cell patch clamp recording,and the conductances of N MDA,AMPA currents in simulated is-chemia neurons were increased over t hose of control neurons. |
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| Keywords: | Excitatory amino acids receptor of i ncreasing excitatory amino acids synapse posterior induce nerve cell injury. |
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